Rashidi Maria R Ward, Snyder Catherine S, Burkhard Kathleen M, Ahmad Raneem, Bhorkar Isha, Mehta Geeta
Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109 USA.
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109 USA.
MRS Commun. 2025;15(3):462-469. doi: 10.1557/s43579-025-00711-2. Epub 2025 Apr 17.
Bone remodeling and immune function are dynamically regulated through cell-cell and cell-matrix interactions by stem and mature cell populations. We investigated the hypothesis that monocytes and pre-osteoblasts respond to cyclic tensile stress and paracrine interactions by differentiating into macrophage-like and osteoblast-like cells. 20% cyclic equibiaxial strain was applied to monocytic U937 and pre-osteoblastic ST2 cells for 72 h. Increased levels of CD11B, CD14, IL-6, and IL-8 in U937 indicated monocytic differentiation. Increased ALP expression and calcium deposition in ST2 indicated differentiation towards osteoblastic lineage. Overall, application of cyclic strain and pre-osteoblastic co-culture induced differentiation in this cyclically strained bone model.
骨重塑和免疫功能通过干细胞和成熟细胞群体之间的细胞-细胞以及细胞-基质相互作用受到动态调节。我们研究了这样一个假说，即单核细胞和前成骨细胞通过分化为巨噬细胞样和成骨细胞样细胞来响应周期性拉伸应力和旁分泌相互作用。对单核细胞U937和前成骨细胞ST2施加20%的周期性等双轴应变，持续72小时。U937中CD11B、CD14、IL-6和IL-8水平的升高表明单核细胞分化。ST2中碱性磷酸酶表达增加和钙沉积表明向成骨细胞谱系分化。总体而言，在这个周期性应变的骨模型中，周期性应变的施加和前成骨细胞共培养诱导了分化。
