Honselmann Genannt Humme Joanna, Dubrowska Kamila, Perużyńska Magdalena, Droździk Marek, Birger Radosław, Jurkiewicz Martyna, Kędzierski Tomasz, Mijowska Ewa, Idzik Tomasz, Sośnicki Jacek G, Filipek Elżbieta, Piz Mateusz, Rakoczy Rafał, Augustyniak Adrian
Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Szczecin, Poland.
Department of Experimental & Clinical Pharmacology, Pomeranian Medical University in Szczecin, Szczecin, Poland.
Appl Microbiol Biotechnol. 2025 Jul 14;109(1):167. doi: 10.1007/s00253-025-13543-w.
Carbon nanotubes (CNTs) emerged as nanomaterials with a wide variety of applications, e.g., as boosters of bioprocesses efficiency. The stimulation of the production of the blue pigment called pyocyanin is one of numerous examples. Moreover, its importance comes from the potential anticancer properties of the pigment. Therefore, this contribution evaluated different commercially available multi-walled carbon nanotubes (MWCNTs) in pyocyanin production using the Design of Experiment methodology. The interactions between pigment-producing bacteria and nanomaterials were revealed as well. Moreover, the purified pigment was tested against normal and cancer cell lines. Interestingly, the results showed that all tested CNTs stimulated pyocyanin production. The most effective CNTs were used in the process optimisation in terms of temperature (32 °C) and carbon nanomaterial concentration (812 μg/mL). It was also revealed that the optical density and viability of the bacterial culture were elevated, while the pyoverdine production was decreased. Furthermore, no oxidative stress was detected. Moreover, the confocal microscopy study indicated that the cells surrounded the aggregates of MWCNT and produced more proteins within the biofilm structure, compared to the control experiment. The tests on neoplastic cell lines showed an excellent antiproliferative activity of pyocyanin against melanoma without pronounced adverse effects on normal fibroblasts. The nanomaterial incorporated in the bioprocess was successfully reused, making the method sustainable and cost-effective. KEY POINTS: • The stimulative effect of nanomaterial on pyocyanin production was optimised • Nanomaterial can be reused in the bioprocess without losing the stimulative effect • Pyocyanin exhibits significant antiproliferative action against melanoma.
碳纳米管（CNTs）作为一种具有广泛应用的纳米材料出现，例如作为生物过程效率的增强剂。刺激名为绿脓菌素的蓝色色素的产生就是众多例子之一。此外，其重要性源于该色素潜在的抗癌特性。因此，本研究采用实验设计方法评估了不同市售多壁碳纳米管（MWCNTs）在绿脓菌素生产中的作用。还揭示了产色素细菌与纳米材料之间的相互作用。此外，对纯化后的色素进行了针对正常细胞系和癌细胞系的测试。有趣的是，结果表明所有测试的碳纳米管都刺激了绿脓菌素的产生。就温度（32°C）和碳纳米材料浓度（812μg/mL）而言，最有效的碳纳米管被用于工艺优化。还发现细菌培养物的光密度和活力有所提高，而绿脓菌素的产量则下降。此外，未检测到氧化应激。此外，共聚焦显微镜研究表明，与对照实验相比，细胞围绕着多壁碳纳米管聚集体，并在生物膜结构内产生了更多蛋白质。对肿瘤细胞系的测试表明，绿脓菌素对黑色素瘤具有出色的抗增殖活性，而对正常成纤维细胞没有明显的不良影响。生物过程中使用的纳米材料成功实现了再利用，使该方法具有可持续性且成本效益高。要点：• 优化了纳米材料对绿脓菌素生产的刺激作用 • 纳米材料可在生物过程中再利用而不丧失刺激作用 • 绿脓菌素对黑色素瘤具有显著的抗增殖作用
