Endemic zoonoses have a dual burden, impacting human and animal health, as well as livelihoods due to livestock losses. One such disease is anthrax, caused by . Data on the burden of anthrax on human and animal health are extremely limited, perpetuating neglect at the policy level in endemic areas and internationally. To address these data inadequacies, we quantified the widespread impacts of anthrax on highly affected communities, focusing on a hyper-endemic area of eastern Africa, the Ngorongoro Conservation Area (NCA) of northern Tanzania. Data were collected between 2016 and 2019 through cross-sectional household surveys, active surveillance, compilation of available hospital and human disease surveillance records, and willingness-to-pay choice experiments conducted within wider socioeconomic surveys. Human anthrax was reported by 34 (16%) of 209 households surveyed. Cases of anthrax in livestock were reported in 63 households (30%), and in all 20 households within Ngoile, the most affected area. The majority of sudden deaths in livestock (368 of 500 cases in 30 months of active surveillance) were confirmed to be caused by anthrax using microscopy or qPCR. Monetary losses due to livestock deaths were significant and during outbreaks commonly exceeded average monthly household income. Despite the dual burden, willingness to adopt anthrax mitigation measures was low. Likely due to resource limitations, households would only consider such measures if they had high efficacy, be it for animals or humans, but even then, price was still an important factor. We discuss the potential benefits that anthrax prevention could have on affected communities, and factors that might influence the success of possible interventions. This study demonstrates that in endemic settings where dependence on livestock productivity is high, the impacts of neglected zoonoses can be diverse, persistent and severe and highlights the challenge of identifying locally acceptable mitigation strategies.