Tian Taiping, Wang Mengqi, Zhu Jingmeng, Sun Yining, Ma Mingjie, Liu Guoliang, Wang Jinshuo, Zhao Bing, Yan Yiping, Wang Yue, Wang Yanli, Li Changyu, Wang Dongyin, Yang Fang, Lu Wenpeng, Xu Peilei
CAAS/Jilin Provincial Key Laboratory of Traditional Chinese Medicinal Materials Cultivation and Propagation, Institute of Special Animal and Plant Sciences, Changchun, China.
College of Agriculture, Yanbian University, Yanji, China.
Front Microbiol. 2025 Jun 30;16:1633730. doi: 10.3389/fmicb.2025.1633730. eCollection 2025.
(Turcz.) Baill. is a widely cultivated medicinal plant with significant economic value and serves as a major component of specialty agriculture in Jilin Province, China. In 2024, a novel basal stem rot disease was observed in Latai Village, Liudaogou Town, Linjiang City, Jilin Province, posing a potential threat to local production.
Field symptoms were characterized by stem-base necrosis, vascular browning, wilting, and plant death, often accompanied by soft rot under high humidity. The causal pathogen was isolated and identified through morphological observation and sequencing of , -tubulin (), and genes. Pathogenicity was confirmed via Koch's postulates on detached stems, seedlings, whole plants, and tissue-cultured plantlets. Biological characteristics were evaluated under different media, temperatures, light conditions, pH levels, and carbon/nitrogen sources. Additionally, seven fungicides (three chemical and four botanical) were tested for inhibitory activity .
The pathogen was identified as , marking the first global report of this species causing basal stem rot on . Optimal growth conditions included CDA or CMA media, 25°C, continuous darkness, and pH 7. Sodium nitrate and lactose were the most favorable nitrogen and carbon sources, respectively. Among the fungicides tested, 98% fluazinam showed the strongest inhibitory effect with an EC₅₀ value of 5.658 mg/L.
This study confirms as a novel and virulent pathogen of in Jilin Province. The findings provide foundational data on its biology and pathogenicity and identify fluazinam as a promising candidate for integrated management of basal stem rot disease.
（Turcz.）Baill. 是一种广泛种植的药用植物，具有重要的经济价值，是中国吉林省特色农业的主要组成部分。2024年，在吉林省临江市六道沟镇腊台村观察到一种新型基腐病，对当地生产构成潜在威胁。
田间症状表现为茎基部坏死、维管束褐变、萎蔫和植株死亡，在高湿度条件下常伴有软腐。通过形态观察以及对β-微管蛋白（β-tubulin）和翻译延伸因子1-α（translation elongation factor 1-alpha，TEF1-α）基因进行测序，分离并鉴定了致病病原体。通过在离体茎、幼苗、整株植物和组培苗上进行柯赫氏法则验证致病性。在不同培养基、温度、光照条件、pH值和碳/氮源下评估生物学特性。此外，测试了七种杀菌剂（三种化学杀菌剂和四种植物源杀菌剂）的抑菌活性。
该病原体被鉴定为[具体名称未给出]，这是该物种首次在全球范围内报道引起[植物名称未给出]基腐病。最佳生长条件包括CDA或CMA培养基、25°C、持续黑暗和pH 7。硝酸钠和乳糖分别是最适宜的氮源和碳源。在所测试的杀菌剂中，98%氟唑菌酰胺表现出最强的抑制作用，EC₅₀值为5.658 mg/L。
本研究证实[具体名称未给出]是吉林省[植物名称未给出]的一种新型致病力强的病原体。研究结果提供了关于其生物学和致病性的基础数据，并确定氟唑菌酰胺是基腐病综合防治的一个有前景的候选药剂。
