Koch H U, Döker R, Fischer W
J Bacteriol. 1985 Dec;164(3):1211-7. doi: 10.1128/jb.164.3.1211-1217.1985.
Toluene-treated Staphylococcus aureus cells did not synthesize teichoic acid and lipoteichoic acid under the conditions used. The organism displayed, however, a high capacity of incorporating D-[14C]alanine into previously formed polymers. The reaction was dependent on ATP and enhanced by magnesium ions. The incorporation rate into lipoteichoic acid correlated with the rate of loss of alanine ester which occurred through transfer to teichoic acid and base-catalyzed hydrolysis. At pH 6.5 the loss (20% within 4 h) was completely compensated for by reesterification. At pH 7.5 the loss was 60%, but by accelerated incorporation it was reduced to 10%. Incorporation was also enhanced when the original substitution of lipoteichoic acid was lowered by previous growth of S. aureus at high salt concentration. The newly added alanine was randomly distributed along the poly(glycerophosphate) chain. The decreased alanine substitution of lipoteichoic acid after growth at high salt concentration was shown to result from a direct inhibition of alanine incorporation.
在所用条件下，经甲苯处理的金黄色葡萄球菌细胞不合成磷壁酸和脂磷壁酸。然而，该微生物显示出将D-[¹⁴C]丙氨酸掺入先前形成的聚合物中的高能力。该反应依赖于ATP，并受到镁离子的增强。掺入脂磷壁酸的速率与通过转移至磷壁酸和碱催化水解而发生的丙氨酸酯损失速率相关。在pH 6.5时，损失（4小时内20%）通过重新酯化得到完全补偿。在pH 7.5时，损失为60%，但通过加速掺入可将其降低至10%。当金黄色葡萄球菌在高盐浓度下预先生长导致脂磷壁酸的原始取代降低时，掺入也会增强。新添加的丙氨酸沿聚（甘油磷酸）链随机分布。高盐浓度下生长后脂磷壁酸丙氨酸取代的降低表明是由于丙氨酸掺入的直接抑制所致。
