采用高效液相色谱法对多烯磷脂分子种类进行定量分析。
Quantitative analysis of polyenoic phospholipid molecular species by high performance liquid chromatography.
作者信息
Takamura H, Narita H, Urade R, Kito M
出版信息
Lipids. 1986 May;21(5):356-61. doi: 10.1007/BF02535701.
The quantitative analysis of phospholipid molecular species containing polyenoic fatty acids is described. Dinitrobenzoyl derivatives of diacylglycerols prepared from phospholipids were separated into individual molecular species by reversed-phase high performance liquid chromatography (HPLC) using a combination of two solvent systems and were quantified at 254 nm. Thirty-six molecular species were resolved from the phosphatidylcholines of rat hearts, human platelets and Chinese hamster V79-R cells. The derivatives of alkenylacyl molecular species from platelet phosphatidylethanolamine were resolved concomitantly with diacyl molecular species.
本文描述了含多烯脂肪酸的磷脂分子种类的定量分析方法。由磷脂制备的二酰基甘油的二硝基苯甲酰衍生物，通过反相高效液相色谱（HPLC），采用两种溶剂系统联用的方式，分离成各个分子种类，并在254nm处进行定量。从大鼠心脏、人血小板和中国仓鼠V79-R细胞的磷脂酰胆碱中分离出了36种分子种类。血小板磷脂酰乙醇胺的烯基酰基分子种类的衍生物与二酰基分子种类同时被分离出来。
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