Brevet A, Plateau P, Best-Belpomme M, Blanquet S
J Biol Chem. 1985 Dec 15;260(29):15566-70.
The unusual bis(5'-nucleosidyl)oligophosphates: Ap4A, Ap4G, Ap3A, and Ap3G, have been measured in cultures of Drosophila cells. Exponentially growing cells contain concentrations of 0.25, 0.31, 0.87, and 2.25 microM, respectively. These nucleotides have been followed after stressing the cells either by CdCl2 addition or by heat-shock treatment. Their concentrations are not affected by exposure to 500 microM CdCl2 during 6 h. Beyond this threshold of cadmium concentration, the nucleotides increase. With 5 mM CdCl2, an enhancement by 2 orders of magnitude of all the dinucleoside tri- and tetra-phosphates is observed. Upon heat-shock from 19 to 37 degrees C, Ap4A, Ap3A, and Ap3G increase up to 2.2, 3, and 3.3 times their initial levels, respectively. The increase is achieved within 1 h.
已在果蝇细胞培养物中测定了异常的双（5'-核苷基）寡磷酸：Ap4A、Ap4G、Ap3A和Ap3G。指数生长的细胞中这些物质的浓度分别为0.25、0.31、0.87和2.25微摩尔。在通过添加CdCl2或热休克处理对细胞施加应激后，对这些核苷酸进行了跟踪监测。在6小时内暴露于500微摩尔CdCl2时，它们的浓度不受影响。超过这个镉浓度阈值，核苷酸会增加。使用5毫摩尔CdCl2时，观察到所有二核苷三磷酸和四磷酸的浓度提高了2个数量级。在从19摄氏度热休克至37摄氏度时，Ap4A、Ap3A和Ap3G分别增加至其初始水平的2.2倍、3倍和3.3倍。这种增加在1小时内实现。
