气相色谱 - 质谱法检测牛脑中的1,4 - 六氢噻嗪 - 3,5 - 二羧酸（环硫氨酸）

Gas-chromatographic mass-spectrometric detection of 1,4-hexahydrothiazepine-3,5-dicarboxylic acid (cyclothionine) in bovine brain.

作者信息

Cavallini D, Pecci L, Matarese R M, Ricci G, Achilli M

出版信息

J Biol Chem. 1985 Dec 15;260(29):15577-9.

Abstract

Cystathionine has been reported to undergo enzymatic changes leading to the formation of seven membered cyclic products (Ricci, G., Santoro, L., Achilli, M., Matarese, R. M., Nardini, M., and Cavallini, D. (1983) J. Biol. Chem. 258, 10511-10517; Cavallini, D., Costa, M., Pensa, B., and Coccia, R. (1985) Biochem. Int. 10, 641-646). Gas-chromatographic and mass-spectrometric evidence reported in this paper indicates that the cyclic derivative of cystathionine, 1,4-hexahydrothiazepine-3,5-dicarboxylic acid, here simply named cyclothionine, is a normal component of bovine brain. This finding together with the detection of the same compound in the urine of cystathioninuric patients (Kodama, H., Sasaki, K., Mikasa, H., Cavallini, D., and Ricci, G. (1984) J. Chromatogr. 311, 183-188) supports the conclusion that cystathionine, apart from its role in trans-sulfuration, is converted also into cyclic compounds whose biochemical significance is as yet unknown.

摘要

据报道，胱硫醚会发生酶促变化，导致形成七元环状产物（里奇，G.，桑托罗，L.，阿基利，M.，马塔雷塞，R.M.，纳尔迪尼，M.，以及卡瓦利尼，D.（1983年）《生物化学杂志》258卷，第10511 - 10517页；卡瓦利尼，D.，科斯塔，M.，彭萨，B.，以及科恰，R.（1985年）《生物化学国际》10卷，第641 - 646页）。本文报道的气相色谱和质谱证据表明，胱硫醚的环状衍生物，1,4 - 六氢噻氮杂卓 - 3,5 - 二羧酸，在此简称为环硫氨酸，是牛脑的正常组成成分。这一发现连同在胱硫醚尿症患者尿液中检测到相同化合物（儿玉，H.，佐佐木，K.，三笠，H.，卡瓦利尼，D.，以及里奇，G.（1984年）《色谱杂志》311卷，第183 - 188页）支持了以下结论：胱硫醚除了在转硫作用中的作用外，还会转化为环状化合物，其生化意义尚不清楚。