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傅里叶变换红外光谱/气相色谱-质谱联用技术在龙舌兰果聚糖代谢快速表征中的应用潜力

Potential of FT-MIR/GC-MS Multivariate Hyphenation for the Fast Characterization of Agavins Metabolism.

作者信息

Salomé-Abarca Luis F, Márquez-López Ruth E, Santiago-García Patricia A, López Mercedes G

机构信息

Colegio de Postgraduados-Campus Montecillo, Posgrado de Recursos Genéticos y Productividad-Fruticultura, Km 36.5 Carretera México - Texcoco, Montecillo, Texcoco de Mora 56230, Estado de México, Mexico.

Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional-Unidad Oaxaca, Oaxaca 71230, Mexico.

出版信息

Anal Chem. 2025 Aug 26;97(33):17954-17961. doi: 10.1021/acs.analchem.5c01074. Epub 2025 Jul 16.

Abstract

Agavins are some of the most recently characterized fructans. FT-MIR is the fastest approach to detect them, and GC-MS provides valuable structural features. Thus, we explored the feasibility of integrating data from these two analytical platforms to characterize the metabolism of agavins from two emblematic agave species, and . FT-MIR quickly provided information about the species and age variation. The analyses demonstrated for the first time the high efficiency of GC-MS glycosidic linkage analysis data for MVDA. Finally, specific MIR regions were correlated to structural features determined through GC-MS, which increased the level of structural information obtained by IR analyses. This approach could accelerate the study of agavins' metabolism.

摘要

龙舌兰果聚糖是最近才被鉴定出来的一些果聚糖。傅里叶变换红外光谱(FT-MIR)是检测它们的最快方法,而气相色谱-质谱联用(GC-MS)则能提供有价值的结构特征。因此,我们探索了整合来自这两个分析平台的数据以表征两种标志性龙舌兰物种龙舌兰果聚糖代谢的可行性。傅里叶变换红外光谱迅速提供了有关物种和年龄变化的信息。分析首次证明了气相色谱-质谱联用糖苷键分析数据用于甲基戊二醇醛(MVDA)的高效性。最后,特定的红外光谱区域与通过气相色谱-质谱联用确定的结构特征相关联,这增加了通过红外分析获得的结构信息水平。这种方法可以加速对龙舌兰果聚糖代谢的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b15/12392261/798707056da8/ac5c01074_0001.jpg

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