Tolba Yasmine M, Ayana Raghda A Abou, Nagui Dina A
Lecturer of Oral Biology, Faculty of Dentistry, Alexandria University, Champlion St., Azarita, Alexandria, Egypt.
Lecturer of Pharmacology and Experimental Therapeutics, Medical Research Institute, Alexandria University, Alexandria, Egypt.
BMC Oral Health. 2025 Jul 16;25(1):1184. doi: 10.1186/s12903-025-06523-z.
This study evaluated the ultrastructural effects of electronic cigarettes' (EC) exposure in rat lingual nerves and muscles and assessed the therapeutic roles of vitamins C, E, and their combination.
Forty adult male albino rats were allocated into 5 groups.
injected saline intraperitoneally, EC group: injected EC-liquid containing nicotine at a dose of 0.75 mg/kg, EC + C group: injected with EC-liquid and then supplemented orally with vitamin C, EC + C group injected with EC-liquid and then supplemented orally with vitamin E, and EC + C&E group: injected with EC-liquid and then supplemented orally with a combination of both vitamins. Transmission electron microscopy (TEM) and oxidative stress biomarkers malondialdehyde (MDA), and superoxide dismutase (SOD) were used to assess tissue damage and antioxidant effects.
EC group showed disrupted myelin sheaths, abnormal mitochondria, elevated MDA, and reduced SOD activity, indicating oxidative damage. EC + C group showed muscular recovery but did not significantly improve oxidative markers (p > 0.05) when compared to EC group. EC + E and EC + C&E groups showed regular myelin sheaths, normal mitochondria, and significant improvements in MDA and SOD levels compared to the EC group (p < 0.01).
Vitamin E alone or combined with vitamin C effectively mitigates EC-induced oxidative stress and ultrastructural damage in muscular and nerve tissues. Vitamin C alone offers insufficient protection, mostly supporting muscular recovery without significantly improving nerve integrity or oxidative status.
本研究评估了电子烟暴露对大鼠舌神经和肌肉的超微结构影响，并评估了维生素C、维生素E及其组合的治疗作用。
将40只成年雄性白化大鼠分为5组。
腹腔注射生理盐水；电子烟组：腹腔注射含0.75mg/kg尼古丁的电子烟液；电子烟+维生素C组：注射电子烟液后口服补充维生素C；电子烟+维生素E组：注射电子烟液后口服补充维生素E；电子烟+维生素C和E组：注射电子烟液后口服补充两种维生素的组合。采用透射电子显微镜（TEM）以及氧化应激生物标志物丙二醛（MDA）和超氧化物歧化酶（SOD）来评估组织损伤和抗氧化作用。
电子烟组显示髓鞘破坏、线粒体异常、MDA升高和SOD活性降低，表明存在氧化损伤。与电子烟组相比，电子烟+维生素C组显示肌肉恢复，但氧化标志物未显著改善（p>0.05）。与电子烟组相比，电子烟+维生素E组和电子烟+维生素C和E组显示髓鞘正常、线粒体正常，MDA和SOD水平显著改善（p<0.01）。
单独使用维生素E或与维生素C联合使用可有效减轻电子烟诱导肌肉和神经组织的氧化应激和超微结构损伤。单独使用维生素C提供的保护不足，主要支持肌肉恢复，而未显著改善神经完整性或氧化状态。
