Gaasbeek Janzen J W, Moorman A F, Lamers W H, Charles R
J Histochem Cytochem. 1985 Dec;33(12):1205-11. doi: 10.1177/33.12.4067274.
Carbamoyl-phosphate synthetase (ammonia) is homogeneously distributed in rat-liver parenchyma at birth, as demonstrated by immunohistochemistry. A heterogeneous distribution can first be demonstrated at 6 days post partum, but can be masked by use of a too sensitive detection system. This heterogeneity is established by a decrease in enzyme content around the hepatic venules and a considerable increase in enzyme content in the remaining parenchyma. The perivenous decrease in enzyme content does not occur in all hepatocytes synchronously. The adult type of heterogeneity is characterized by a perivenous layer, only two to three cells thick, in which carbamoyl-phosphate synthetase can no longer be detected, irrespective of the sensitivity of the assay used. This situation is fully established at the age of two months.
免疫组织化学结果表明，氨甲酰磷酸合成酶（氨）在出生时均匀分布于大鼠肝脏实质中。产后6天时首次可显示出其分布不均一性，但使用过于灵敏的检测系统可能会掩盖这一现象。这种不均一性是由肝静脉周围酶含量的减少以及其余实质中酶含量的显著增加所导致的。并非所有肝细胞的静脉周围酶含量都会同步减少。成年期的不均一性特征是存在一层仅两到三个细胞厚的静脉周围层，无论所采用检测方法的灵敏度如何，在该层中均无法检测到氨甲酰磷酸合成酶。这种情况在两个月大时完全形成。
