Multhaupt H, Fritz P, Schumacher K
Department of Haematology, Robert-Bosch-Krankenhaus, Stuttgart, Federal Republic of Germany.
Histochemistry. 1987;87(5):465-70. doi: 10.1007/BF00496818.
Monoclonal antibodies against human liver arginase were raised in order to determine the exact distribution of arginase in human liver using a modified indirect unlabelled immunoperoxidase method. In normal human liver specific immunohistochemical staining was found in the cytoplasm of hepatocytes. Portal components (bile ducts and veins) and fibrous tissue were non-reactive, while erythrocytes were slightly positive. The specificity of the immunological reaction was confirmed by control tests. Spectrophotometry was used to quantitate the immunohistochemical reaction product, and the results indicated that arginase is homogeneously distributed in the liver lobule.
为了使用改良的间接未标记免疫过氧化物酶方法确定精氨酸酶在人肝脏中的精确分布，制备了抗人肝脏精氨酸酶的单克隆抗体。在正常人肝脏中，在肝细胞的细胞质中发现了特异性免疫组织化学染色。门静脉成分（胆管和静脉）和纤维组织无反应，而红细胞呈弱阳性。对照试验证实了免疫反应的特异性。使用分光光度法定量免疫组织化学反应产物，结果表明精氨酸酶在肝小叶中均匀分布。
