Suppr超能文献

体外报告良好标准指南(GIVReSt)——供利益相关者讨论的草案及背景文件。

Guidance for Good In Vitro Reporting Standards (GIVReSt) - A draft for stakeholder discussion and background documentation.

作者信息

Mohapatra Ronit, Leist Marcel, von Aulock Sonja, Hartung Thomas

机构信息

Center for Alternatives to Animal Testing (CAAT), Johns Hopkins University, Baltimore, MD, USA.

CAAT-Europe, University of Konstanz, Konstanz, Germany.

出版信息

ALTEX. 2025;42(3):376-396. doi: 10.14573/altex.2507041.

Abstract

Reproducibility of cell culture experiments between laboratories needs to be improved by ensuring more complete reporting of methodology in scientific papers. The minimum reporting standards sug-gested here cover various cell culture methods including monolayers, stem cells, organoids, and microphysiological systems (MPS). The standards build on existing guidance like Good Cell Culture Practice (GCCP 2.0) and OECD test reporting guidelines on how to quality-assure in vitro work, focusing on transparency and completeness of reporting. Key elements to be reported include full details of cell source and identity, cell quality control and characterization, materials and reagents used, culture conditions and protocols, experimental design, data analysis, data availability, and legal and ethical aspects. For complex models, additional details need to be provided such as cell ratios, microenvironment conditions, functional characterization, etc. The guidance for Good In Vitro Reporting Standards (GIVReSt) is part of a broader initiative of evidence-based toxicology encompassing the improvement of the quality of in vitro studies for safety assessments and regulatory decisions. In summary, GIVReSt addresses incomplete reporting as a major factor affecting repro-ducibility of cell culture experiments by providing clear standards around transparency and rigor in reporting. The integration of agentic artificial intelligence (AI) is envisioned to streamline compliance checking, providing real-time feedback and accelerating scientific discovery by making high-quality evidence more accessible. This should lead to more reliable cell culture research overall.

摘要

通过确保科学论文中更完整地报告方法,实验室间细胞培养实验的可重复性需要得到提高。此处建议的最低报告标准涵盖了各种细胞培养方法,包括单层培养、干细胞、类器官和微生理系统(MPS)。这些标准基于现有的指南,如良好细胞培养规范(GCCP 2.0)和经合组织关于如何对体外工作进行质量保证的测试报告指南,重点关注报告的透明度和完整性。需要报告的关键要素包括细胞来源和身份的全部细节、细胞质量控制和表征、所用材料和试剂、培养条件和方案、实验设计、数据分析、数据可用性以及法律和伦理方面。对于复杂模型,需要提供额外的细节,如细胞比例、微环境条件、功能表征等。良好体外报告标准指南(GIVReSt)是循证毒理学更广泛倡议的一部分,该倡议包括提高体外研究质量以进行安全性评估和监管决策。总之,GIVReSt通过围绕报告的透明度和严谨性提供明确标准,将不完整报告视为影响细胞培养实验可重复性的主要因素。预计整合智能人工智能(AI)将简化合规检查,提供实时反馈并通过使高质量证据更易获取来加速科学发现。这总体上应会带来更可靠的细胞培养研究。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验