Seifi Sina, Ghaee Azadeh
Faculty of Life Science Engineering, College of Interdisciplinary Science & Technologies, University of Tehran, P.O. Box: 143951374, Tehran, Iran.
Faculty of Life Science Engineering, College of Interdisciplinary Science & Technologies, University of Tehran, P.O. Box: 143951374, Tehran, Iran.
Int J Biol Macromol. 2025 Aug;320(Pt 3):145904. doi: 10.1016/j.ijbiomac.2025.145904. Epub 2025 Jul 15.
In this study, a novel nanocomposite scaffold comprising pectin, gelatin, and amoxicillin trihydrate-loaded UiO-66-NH metal-organic frameworks (UiO-66-NH@AMX) was fabricated for skin tissue engineering applications. UiO-66-NH nanoparticles were synthesized hydrothermally and subsequently loaded with amoxicillin trihydrate. Finally, UiO-66-NH@AMX nanoparticles were incorporated into pectin-gelatin scaffolds using the freeze-drying method. FE-SEM imaging confirmed the octahedral morphology of the nanoparticles before and after drug loading. FTIR analysis demonstrated successful drug incorporation via non-covalent interactions. DLS and zeta potential measurements showed that UiO-66-NH nanoparticles had a mean size of 218.5 ± 14 nm and a surface charge of +44.1 ± 2.7 mV. After drug loading, the size slightly increased to 248.8 ± 19 nm, and the surface charge decreased to +32.6 ± 2.5 mV, while maintaining colloidal stability. All of the scaffolds showed porous structures with interconnected pores in SEM imaging. The incorporation of UiO-66-NH@AMX nanoparticles into the pectin-gelatin scaffolds up to 3 % (w/w) increased the tensile strength and Young's modulus of the scaffolds 37.6 % and 56.7 %, respectively. Antibacterial activity was evaluated by CFU assay, where UiO-66-NH@AMX containing scaffolds revealed significant bacterial reduction against E. coli and S. aureus colonies. MTT, cell adhesion, and cell scratch assays conducted on L929 fibroblasts. All of the scaffolds demonstrated >85 % cell viability after 3 days of culture. Furthermore, scaffolds with 3 % (w/w) UiO-66-NH@AMX enhanced cell adhesion and increased migration rate in scratch closure assay. Overall, the results indicate that the novel nanocomposite scaffolds are promising candidates for skin tissue engineering and wound healing applications.
在本研究中，制备了一种新型纳米复合支架，其由果胶、明胶和负载三水合阿莫西林的UiO-66-NH金属有机框架（UiO-66-NH@AMX）组成，用于皮肤组织工程应用。通过水热法合成UiO-66-NH纳米颗粒，随后负载三水合阿莫西林。最后，采用冷冻干燥法将UiO-66-NH@AMX纳米颗粒掺入果胶-明胶支架中。场发射扫描电子显微镜（FE-SEM）成像证实了载药前后纳米颗粒的八面体形态。傅里叶变换红外光谱（FTIR）分析表明通过非共价相互作用成功载入了药物。动态光散射（DLS）和zeta电位测量表明，UiO-66-NH纳米颗粒的平均尺寸为218.5±14nm，表面电荷为+44.1±2.7mV。载药后，尺寸略有增加至248.8±19nm，表面电荷降至+32.6±2.5mV，同时保持胶体稳定性。所有支架在扫描电子显微镜成像中均显示出具有相互连通孔隙的多孔结构。将UiO-66-NH@AMX纳米颗粒以高达3%（w/w）的比例掺入果胶-明胶支架中，分别使支架的拉伸强度和杨氏模量提高了37.6%和56.7%。通过菌落形成单位（CFU）测定评估抗菌活性，其中含UiO-66-NH@AMX的支架对大肠杆菌和金黄色葡萄球菌菌落显示出显著的细菌减少。对L929成纤维细胞进行了MTT、细胞黏附及细胞划痕试验。培养3天后，所有支架的细胞活力均>85%。此外，含3%（w/w）UiO-66-NH@AMX的支架在划痕闭合试验中增强了细胞黏附并提高了迁移率。总体而言，结果表明这种新型纳米复合支架是皮肤组织工程和伤口愈合应用的有前景的候选材料。
