Wang Linna, Jiang Nianqing, Zou Lina, He Jinhua, Yao Zixian, Zhang Shunran, Xiang Yan
Anhui Provincial Key Laboratory of Forest Resources and Silviculture, Anhui Agricultural University, Hefei, 230036, China.
BMC Plant Biol. 2025 Jul 17;25(1):923. doi: 10.1186/s12870-025-06849-4.
Pecan (Carya illinoinensis) is an economically and ecologically important tree species, but its growth is frequently challenged by salt stress and heat stress. bZIP transcription factors are key regulators of plant responses to abiotic stresses. This study aimed to functionally characterize the pecan transcription factor CibZIP43 and reveal its role in salt and heat stress tolerance. The CibZIP43 gene was overexpressed in Arabidopsis, and the results showed that CibZIP43 overexpression (OE) in Arabidopsis significantly enhanced the tolerance of transgenic plants to salt and heat stress. Physiological and biochemical analyzes showed that CibZIP43 OE lines exhibited better growth and improved physiological indices under salt and heat stress, including increased chlorophyll content, relative water content, and antioxidant enzyme activities, along with reduced membrane lipid peroxidation and proline accumulation. Moreover, CibZIP43 OE enhanced plant adaptation to salt and heat stress by regulating the expression of Na/K transporter-related and heat stress-related genes. These findings uncover new molecular mechanisms and provide potential genetic resources for breeding for stress-tolerance in pecan varieties.
山核桃（Carya illinoinensis）是一种具有重要经济和生态价值的树种，但其生长经常受到盐胁迫和热胁迫的挑战。bZIP转录因子是植物对非生物胁迫响应的关键调节因子。本研究旨在对山核桃转录因子CibZIP43进行功能鉴定，并揭示其在耐盐和耐热胁迫中的作用。CibZIP43基因在拟南芥中过表达，结果表明，拟南芥中CibZIP43过表达（OE）显著增强了转基因植物对盐胁迫和热胁迫的耐受性。生理生化分析表明，CibZIP43 OE株系在盐胁迫和热胁迫下表现出更好的生长和改善的生理指标，包括叶绿素含量增加、相对含水量和抗氧化酶活性提高，同时膜脂过氧化和脯氨酸积累减少。此外，CibZIP43 OE通过调节Na/K转运蛋白相关基因和热胁迫相关基因的表达增强了植物对盐胁迫和热胁迫的适应性。这些发现揭示了新的分子机制，并为山核桃品种耐胁迫育种提供了潜在的遗传资源。
