使用纳米孔-illumina混合测序法对伏马菌素B1转化细菌FS4_11的全基因组序列数据进行分析。

Whole genome sequence data of FS4_11, a fumonisin B1-transforming bacterium, using hybrid nanopore-illumina sequencing.

作者信息

Wang Yang, Zhao Mengru, Wang Zhe, Luo Xiaohong, Wang Chengfei, Guo Baoyuan

机构信息

Institute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China.

School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

出版信息

Data Brief. 2025 Jun 24;61:111829. doi: 10.1016/j.dib.2025.111829. eCollection 2025 Aug.

DOI:10.1016/j.dib.2025.111829

PMID:40677273

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12269614/

Abstract

The genome of Comamonas sediminis FS4_11, a bacterial strain with mycotoxin fumonisin B1 (FB1) transformation capability, was sequenced using Oxford Nanopore Technologies (ONT) and Illumina platforms. The final assembly generated a circular chromosome of 5,148,490 bp with a mean G+C content of 63.74%, representing a contiguous genomic structure. Genome annotation predicted 4565 protein-coding sequences (CDSs), 82 transfer RNAs (tRNAs), 18 ribosomal RNAs (rRNAs; 6 each of 5S, 16S, and 23S rRNA), 1 transfer-messenger RNA (tmRNA), and 8 pseudogenes and other non-coding RNAs. Functional annotation identified 939 potential virulence factors, two putative AdeF-related antibiotic resistance genes, 1486 potential pathogen-host interaction proteins, and a candidate carboxylesterase for FB1 transformation. This dataset primarily aids in identifying potential FB1 detoxification enzyme genes and assessing strain biosafety. It also offers significant reuse potential for comparative genomics and understanding bacterial evolution.

摘要

利用牛津纳米孔技术（ONT）和Illumina平台对具有霉菌毒素伏马菌素B1（FB1）转化能力的细菌菌株嗜泥丛毛单胞菌FS4_11的基因组进行了测序。最终组装得到了一条5,148,490 bp的环状染色体，平均G+C含量为63.74%，呈现出连续的基因组结构。基因组注释预测有4565个蛋白质编码序列（CDS）、82个转运RNA（tRNA）、18个核糖体RNA（rRNA；5S、16S和23S rRNA各6个）、1个转运信使RNA（tmRNA）以及8个假基因和其他非编码RNA。功能注释鉴定出939个潜在毒力因子、两个假定的与AdeF相关的抗生素抗性基因、1486个潜在的病原体-宿主相互作用蛋白以及一个用于FB1转化的候选羧酸酯酶。该数据集主要有助于鉴定潜在的FB1解毒酶基因并评估菌株生物安全性。它还为比较基因组学和理解细菌进化提供了显著的重用潜力。