Özuygur Ermis Saliha Selin, Malmhäll Carina, Borres Magnus P, Movérare Robert, Lisik Daniil, Abohalaka Reshed, Ercan Selin, Schmeisser Susanne, Basna Rani, Mincheva Roxana, Wennergren Göran, Lötvall Jan, Ekerljung Linda, Rådinger Madeleine, Kankaanranta Hannu, Nwaru Bright I
Krefting Research Centre, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
J Allergy Clin Immunol Glob. 2025 Jun 6;4(3):100510. doi: 10.1016/j.jacig.2025.100510. eCollection 2025 Aug.
Eosinophil-derived neurotoxin (EDN), an eosinophil granule protein, is a candidate biomarker in asthma to reflect eosinophilic activation.
We sought to characterize the distribution of serum and nasal lavage fluid (NLF) EDN levels in different adult population subgroups and assess the determinants of high EDN levels.
Serum and NLF samples were collected from a population-based adult cohort study, the West Sweden Asthma Study. In total, serum EDN was measured in 2939 subjects and NLF EDN in 878 subjects, both using ImmunoCAP (Phadia AB/Thermo Fisher Scientific, Uppsala, Sweden). High EDN levels were defined as values higher than the third quartile, derived from randomly selected general adult population. Background and clinical determinants of high EDN levels were assessed in serum and NLF samples.
Males had higher EDN levels than females, regardless of presence of asthma or atopy. EDN levels differed by obesity and atopy, but not by age, in the random adult population. Obesity was associated with high serum EDN levels in females (adjusted odds ratio [AOR], 1.81; 95% CI, 1.08-3.04) but not in males (AOR, 1.20; 95% CI, 0.63-2.29) in the random adult population. Subjects with current asthma were more likely to have higher serum EDN levels (AOR, 1.83; 95% CI, 1.55-2.17) than those without current asthma, but not higher NLF EDN levels.
Adults with current asthma were more likely to have higher serum, but not NLF, EDN levels than those without asthma. Although serum EDN could be a promising marker in asthma, background determinants-such as sex, obesity, and atopy-should be considered when interpreting EDN levels.
嗜酸性粒细胞衍生神经毒素（EDN）是一种嗜酸性粒细胞颗粒蛋白，是哮喘中反映嗜酸性粒细胞活化的候选生物标志物。
我们试图描述不同成年人群亚组中血清和鼻灌洗液（NLF）EDN水平的分布情况，并评估EDN高水平的决定因素。
血清和NLF样本来自一项基于人群的成年队列研究——瑞典西部哮喘研究。总共对2939名受试者检测了血清EDN，对878名受试者检测了NLF EDN，均使用免疫捕获法（瑞典乌普萨拉的法迪亚公司/赛默飞世尔科技公司）。EDN高水平定义为高于随机选择的普通成年人群的第三个四分位数的值。在血清和NLF样本中评估EDN高水平的背景和临床决定因素。
无论是否患有哮喘或特应性疾病，男性的EDN水平均高于女性。在随机抽取的成年人群中，EDN水平因肥胖和特应性疾病而有所不同，但不受年龄影响。在随机抽取的成年人群中，肥胖与女性血清EDN高水平相关（校正比值比[AOR]，1.81；95%置信区间[CI]，1.08 - 3.04），但与男性无关（AOR，1.20；95% CI，0.63 - 2.29）。与无当前哮喘的受试者相比，当前患有哮喘的受试者血清EDN水平更高（AOR，1.83；95% CI，1.55 - 2.17），但NLF EDN水平并不更高。
与无哮喘的成年人相比，当前患有哮喘的成年人血清EDN水平更可能更高，但NLF EDN水平并非如此。尽管血清EDN可能是哮喘中有前景的标志物，但在解释EDN水平时应考虑诸如性别、肥胖和特应性疾病等背景决定因素。
