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莱茵衣藻短鞭毛突变体的鞭毛形态

Flagellar morphology in stumpy-flagella mutants of Chlamydomonas reinhardtii.

作者信息

Jarvik J W, Chojnacki B

出版信息

J Protozool. 1985 Nov;32(4):649-56. doi: 10.1111/j.1550-7408.1985.tb03095.x.

DOI:10.1111/j.1550-7408.1985.tb03095.x
PMID:4067877
Abstract

Sixteen new mutants of the biflagellate green alga Chlamydomonas reinhardtii with either stumpy-flagella or no flagella at all were examined by electron microscopy. Four of the mutants were found to carry short bulbous flagella containing amorphous electron-dense material which may represent unassembled flagellar protein. Basal bodies of normal ultrastructure were present in all mutants. Dikaryon dominance tests indicated that the stumpy mutations were recessive to wild-type in all cases tested. Stumpy mutations also conferred a measure of detergent resistance to Chlamydomonas, apparently by affecting the detergent-solubility of the flagellar membrane.

摘要

利用电子显微镜对莱茵衣藻这种双鞭毛绿藻的16种新突变体进行了研究,这些突变体要么有短粗型鞭毛,要么完全没有鞭毛。发现其中4种突变体带有短的球根状鞭毛,其中含有无定形的电子致密物质,这可能代表未组装的鞭毛蛋白。所有突变体中都存在超微结构正常的基体。双核体显性测试表明,在所有测试的情况下,短粗型突变相对于野生型都是隐性的。短粗型突变显然通过影响鞭毛膜的去污剂溶解性,也赋予了衣藻一定程度的耐去污剂能力。

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1
Flagellar morphology in stumpy-flagella mutants of Chlamydomonas reinhardtii.莱茵衣藻短鞭毛突变体的鞭毛形态
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