利用莱茵衣藻鞭毛数量可变的突变体分析鞭毛大小控制。
Analysis of flagellar size control using a mutant of Chlamydomonas reinhardtii with a variable number of flagella.
作者信息
Kuchka M R, Jarvik J W
出版信息
J Cell Biol. 1982 Jan;92(1):170-5. doi: 10.1083/jcb.92.1.170.
Abstract
A mutant of Chlamydomonas reinhardtii with a variable number of flagella per cell has been used to investigate flagellar size control. The mutant and wild-type do not differ in cell size nor in flagellar length, yet the size of the intracellular pool of flagellar precursor protein can differ dramatically among individual mutant cells, with, for example, triflagellate cells having three times the pool of monoflagellate cells. Because cells of the same size, but with very different pool sizes, have flagella of identical length, it appears that the concentration of the unassembled flagellar precursor protein pool does not regulate flagellar length. The relation between cell size, pool size, and flagellar length has also been investigated for wild-type cells of different sizes and ploidies. Again, flagellar length appears to be maintained independent of pool size or concentration.
摘要
一种每个细胞鞭毛数量可变的莱茵衣藻突变体已被用于研究鞭毛大小控制。该突变体和野生型在细胞大小和鞭毛长度上没有差异，但鞭毛前体蛋白的细胞内库大小在单个突变体细胞之间可能有显著差异，例如，三鞭毛细胞的库是单鞭毛细胞的三倍。由于大小相同但库大小差异很大的细胞具有相同长度的鞭毛，因此似乎未组装的鞭毛前体蛋白库的浓度并不调节鞭毛长度。对于不同大小和倍性的野生型细胞，也研究了细胞大小、库大小和鞭毛长度之间的关系。同样，鞭毛长度似乎保持独立于库大小或浓度。
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本文引用的文献
1
Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff.紫外线诱导的莱茵衣藻（Chlamydomonas moewusii Gerloff）突变
J Gen Microbiol. 1952 May;6(3-4):233-48. doi: 10.1099/00221287-6-3-4-233.
2
A theory of linear and helical aggregations of macromolecules.大分子的线性和螺旋聚集体理论。
J Mol Biol. 1962 Jan;4:10-21. doi: 10.1016/s0022-2836(62)80112-0.
3
THE CONTROL OF GAMETIC DIFFERENTIATION IN LIQUID CULTURES OF CHLAMYDOMONAS.莱茵衣藻液体培养中配子分化的控制
J Cell Comp Physiol. 1964 Apr;63:157-64. doi: 10.1002/jcp.1030630204.
4
The genetics and cytology of Chlamydomonas.衣藻的遗传学与细胞学
Annu Rev Microbiol. 1960;14:197-216. doi: 10.1146/annurev.mi.14.100160.001213.
5
Relation between cell growth and cell division. III. Changes in nuclear volume and growth rate and prevention of cell division in Amoeba proteus resulting from cytoplasmic amputations.细胞生长与细胞分裂的关系。III. 变形虫细胞质切断后细胞核体积、生长速率的变化及细胞分裂的抑制
Exp Cell Res. 1956 Aug;11(1):94-8. doi: 10.1016/0014-4827(56)90193-8.
6
Mutants of Chlamydomonas moewusii with impaired motility.运动能力受损的莱茵衣藻突变体。
J Gen Microbiol. 1954 Dec;11(3):358-63. doi: 10.1099/00221287-11-3-358.
7
Nutritional studies with Chlamydomonas reinhardi.莱茵衣藻的营养研究。
Ann N Y Acad Sci. 1953 Oct 14;56(5):831-8. doi: 10.1111/j.1749-6632.1953.tb30261.x.
8
Oversized flagellar membrane protein in paralyzed mutants of Chlamydomonas reinhardrii.莱茵衣藻瘫痪突变体中的超大鞭毛膜蛋白。
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9
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J Cell Biol. 1981 Jan;88(1):80-8. doi: 10.1083/jcb.88.1.80.
10
Increased levels of mRNAs for tubulin and other flagellar proteins after amputation or shortening of Chlamydomonas flagella.衣藻鞭毛被截断或缩短后，微管蛋白和其他鞭毛蛋白的信使核糖核酸水平升高。
Cell. 1980 Jun;20(2):469-77. doi: 10.1016/0092-8674(80)90633-9.
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