Ma Longfei, Liu Huijuan, Ge Zhongyu, Bai Bing, Zhao Jianle, Chen Shun, Zhou Binbin, Zhang Jiaqi, Wang Tian, Wang Chao
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
J Therm Biol. 2025 Jul;131:104204. doi: 10.1016/j.jtherbio.2025.104204. Epub 2025 Jul 3.
Heat stress (HS) leads to abnormal lipid metabolism in broilers, while rutin as a natural flavonoid with multiple biological functions shows potential to regulate antioxidant capacity and lipid metabolism. Therefore, this study aimed to investigate the effects of rutin supplementation on antioxidant and lipid metabolism functions in heat-stress broilers and to further explore its potential mechanisms. A total of 192 male broilers were randomly divided into three groups and treated with normal control (CON), heat stress (34 °C, HS), and HS with 500 mg kg rutin supplementation (HS + Rutin), respectively. The results showed that dietary supplementation of 500 mg kg rutin could significantly reverse the HS-induced liver damage. Rutin also mitigated HS-induced increases in liver triglyceride content and decreases in high-density lipoprotein and fatty acid binding protein contents. This might be achieved by increasing the expression of PPARα and decreasing the expression of SREBP1, which regulated the expression of downstream factors (such as CPT1, ACOX1, and ACC). Compared with the HS group, rutin increased mitochondrial DNA copy number and maintained mitochondrial homeostasis by regulating the expression of related genes (such as AMPK, PGC1-α, and SIRT3). In addition, rutin increased the activity of antioxidant enzymes, decreased the content of malondialdehyde, and activated the nuclear respiratory factor 2 (Nrf2) signaling pathway in the liver, which alleviated HS-induced oxidative stress. The effect of rutin in alleviating abnormal lipid deposition in HS broiler livers were correlated with indicators that reflected the antioxidant capacity and mitochondrial homeostasis. In conclusion, dietary 500 mg kg rutin alleviates heat stress-induced hepatic abnormal lipid metabolism of broilers via improving antioxidant capacity to maintain mitochondrial homeostasis.
热应激（HS）会导致肉鸡脂质代谢异常，而芦丁作为一种具有多种生物学功能的天然黄酮类化合物，显示出调节抗氧化能力和脂质代谢的潜力。因此，本研究旨在探讨补充芦丁对热应激肉鸡抗氧化和脂质代谢功能的影响，并进一步探索其潜在机制。总共192只雄性肉鸡被随机分为三组，分别进行正常对照（CON）、热应激（34℃，HS）和补充500mg/kg芦丁的热应激处理（HS+芦丁）。结果表明，日粮中添加500mg/kg芦丁可显著逆转热应激诱导的肝脏损伤。芦丁还减轻了热应激诱导的肝脏甘油三酯含量增加以及高密度脂蛋白和脂肪酸结合蛋白含量降低。这可能是通过增加PPARα的表达和降低SREBP1的表达来实现的，PPARα和SREBP1调节下游因子（如CPT1、ACOX1和ACC）的表达。与热应激组相比，芦丁通过调节相关基因（如AMPK、PGC1-α和SIRT3）的表达增加了线粒体DNA拷贝数并维持了线粒体稳态。此外，芦丁增加了抗氧化酶的活性，降低了丙二醛含量，并激活了肝脏中的核呼吸因子2（Nrf2）信号通路，从而减轻了热应激诱导的氧化应激。芦丁减轻热应激肉鸡肝脏异常脂质沉积的作用与反映抗氧化能力和线粒体稳态的指标相关。总之，日粮中添加500mg/kg芦丁通过提高抗氧化能力以维持线粒体稳态，减轻了热应激诱导的肉鸡肝脏脂质代谢异常。
