McCarey B E, al Reaves T
Department of Ophthalmology, Emory University, Atlanta, GA 30322, USA.
Curr Eye Res. 1995 Jun;14(6):505-10. doi: 10.3109/02713689509003762.
The Fluorotron Master fluorophotometer was used to measure the corneal epithelial permeability to carboxyfluorescein in the normal unanesthetized rabbit. This was related to the epithelial fluorescein staining grade as assessed with a slit lamp. The carboxyfluorescein concentration in the cornea was measured at 3, 60 and 120 min after bathing the cornea in 2.7 x 10(-3) M carboxyfluorescein for 5 min. The epithelial cell layer permeability was calculated from the corneal carboxyfluorescein concentration immediately after a balanced salt solution rinse (initial technique) and from the time zero intercept value extrapolated from the 60 and 120 min data (intercept technique). The slit lamp demonstrated 69% of the nonpaired rabbit eyes were free of epithelial defects, whereas, 46% of the paired rabbit eyes were free of defects. Epithelial permeability values determined from the initial or intercept technique were similar (p = 0.77, unpaired 2 tailed t-test for data < 0.165 nm/s). The initial analysis permeability values ranged from 0.0154 to 4.309 nm/s., n = 29 There was a Gaussian distribution of data between 0.015 and 0.144 nm/s with a mean of 0.0646 +/- 0.0070 nm/s. The larger values correlated with the naturally occurring epithelial defects observed with the slit lamp. The epithelial permeability values were independent of the contralateral eye or subsequent day measurements.
使用Fluorotron Master荧光光度计测量正常未麻醉兔角膜上皮对羧基荧光素的通透性。这与用裂隙灯评估的上皮荧光素染色等级相关。在将角膜置于2.7×10⁻³ M羧基荧光素中5分钟后，于3、60和120分钟测量角膜中的羧基荧光素浓度。上皮细胞层通透性通过平衡盐溶液冲洗后立即测量的角膜羧基荧光素浓度（初始技术）以及从60和120分钟数据外推的时间零截距值（截距技术）来计算。裂隙灯显示69%的未配对兔眼无上皮缺损，而配对兔眼中46%无缺损。通过初始或截距技术确定的上皮通透性值相似（p = 0.77，对数据<0.165 nm/s进行未配对双尾t检验）。初始分析的通透性值范围为0.0154至4.309 nm/s，n = 29。在0.015至0.144 nm/s之间数据呈高斯分布，平均值为0.0646±0.0070 nm/s。较大的值与裂隙灯观察到的自然发生的上皮缺损相关。上皮通透性值与对侧眼或后续日期的测量无关。
