Boot R, Koopman J P, Kruijt B C, Lammers R M, Kennis H M, Lankhorst A, Mullink J W, Stadhouders A M, De Boer H, Welling G W
Lab Anim. 1985 Oct;19(4):344-52. doi: 10.1258/002367785780887428.
Hysterectomy-derived germ-free rabbits were given colonization-resistant caecal flora (CRF) from mice, or microflora obtained from the caecum of an antibiotic-decontaminated conventional rabbit and compared with rabbits conventionally raised with the doe. Bodyweight and the following intestinal parameters were determined for the 3 groups: colonization resistance to E. coli, relative caecal weight, villus:crypt ratio (ileum), beta-aspartylglycine (faeces), volatile fatty acids (caecum), and bile acids (faeces). Germ-free rabbits given mouse CRF-flora showed values quite different from control animals for most parameters, indicating unsuitability of mouse CRF flora to 'normalize' rabbits. In germ-free rabbits given modified (antibiotic-treated) rabbit flora, values for most parameters were intermediate between those found for the other 2 groups. This species-specific caecal flora should be improved to provide full 'normalization' of germ-free rabbits.
将子宫切除术后的无菌兔用来自小鼠的具有定植抗性的盲肠菌群（CRF）进行定植，或用从经抗生素净化的常规兔盲肠中获得的微生物群进行定植，并与由母兔常规饲养的兔进行比较。测定了这3组兔的体重以及以下肠道参数：对大肠杆菌的定植抗性、相对盲肠重量、绒毛：隐窝比（回肠）、β-天冬氨酰甘氨酸（粪便）、挥发性脂肪酸（盲肠）和胆汁酸（粪便）。接受小鼠CRF菌群定植的无菌兔在大多数参数上显示出与对照动物截然不同的值，表明小鼠CRF菌群不适用于使兔“正常化”。在接受改良（抗生素处理）兔菌群定植的无菌兔中，大多数参数的值介于其他两组之间。应改进这种物种特异性的盲肠菌群，以使无菌兔完全“正常化”。
