Smirnova O V, Vishnyakova T G, Smirnov A N, Rozen V B
J Steroid Biochem. 1985 Oct;23(4):461-8. doi: 10.1016/0022-4731(85)90193-1.
The number of estradiol (E2) binding sites of rat liver unusual estrogen-binding protein (NUEBP) was measured, using a novel modification of the quantitative method of specific UEBP determination. In liver cytosol of mature male and female rats, NUEBP amounted to 6.83 +/- 0.49 and less than 0.05 pmol/mg protein, respectively. Neonatal administration of testosterone-propionate (TP) and TP injections at later periods of ontogenesis increased NUEBP in female rat liver in a similar fashion. The elevated NUEBP was found in the liver of mature ovariectomized females 30 days after cessation of TP injections. Hypophysectomy (but not adrenalectomy or thyroidectomy) prevented TP induction of elevated NUEBP in pubertal females. E2 injections reversibly decreased NUEBP in the liver of all animals under study except of hypophysectomized males. A stimulating regulatory effect of TP on NUEBP in male rat liver was observed only in the case of endogenous androgen deficiency and low NUEBP. TP prevented the E2-dependent decrease of NUEBP upon their simultaneous injections and increased the E2-reduced NUEBP when injected after E2. Hypophysectomy led to a decrease of NUEBP in pubertal males but only slightly affected that in castrated animals. After TP injections to hypophysectomized males, NUEBP returned to a level next to the initial one. It was concluded that estrogen-androgen regulation of the UEBP level led to the maintenance of sex differences in the UEBP content.
采用一种测定特异性非典型雌激素结合蛋白（NUEBP）定量方法的新改良法，测量了大鼠肝脏中雌二醇（E2）结合位点的数量。在成年雄性和雌性大鼠的肝细胞溶胶中，NUEBP分别为6.83±0.49和低于0.05 pmol/mg蛋白质。新生期给予丙酸睾酮（TP）以及在个体发育后期注射TP，均以类似方式增加了雌性大鼠肝脏中的NUEBP。在停止注射TP 30天后，在成熟去卵巢雌性大鼠的肝脏中发现NUEBP升高。垂体切除（而非肾上腺切除或甲状腺切除）可防止青春期雌性大鼠中TP诱导的NUEBP升高。除垂体切除的雄性大鼠外，E2注射可使所有研究动物肝脏中的NUEBP可逆性降低。仅在内源性雄激素缺乏且NUEBP较低的情况下，观察到TP对雄性大鼠肝脏中NUEBP具有刺激调节作用。TP在与E2同时注射时可防止E2依赖性的NUEBP降低，在E2注射后注射TP可使E2降低的NUEBP升高。垂体切除导致青春期雄性大鼠的NUEBP降低，但对去势动物的影响较小。对垂体切除的雄性大鼠注射TP后，NUEBP恢复到接近初始水平。得出的结论是，雌激素 - 雄激素对NUEBP水平的调节导致了NUEBP含量的性别差异得以维持。
