Hanafiah Muhammad, Ferasyi Teuku Reza, Rahmi Erdiansyah, Winaruddin Winaruddin, Dewi Kartika, Saputri Roliamy, Redukmi Nisrima
Laboratory of Parasitology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Laboratory Veterinary Public Health of Universitas Syiah Kuala, Banda Aceh, Indonesia.
Vet World. 2025 Jun;18(6):1527-1539. doi: 10.14202/vetworld.2025.1527-1539. Epub 2025 Jun 15.
Long-tailed macaques () serve as critical sentinels for zoonotic disease surveillance due to their ecological proximity to human populations. Understanding their parasitic burden is vital for conservation and public health, particularly in ecotourism areas where human-primate interactions are frequent. This study aimed to investigate the prevalence, diversity, and morphological characteristics of gastrointestinal (GI) and ectoparasites in across four natural habitats in Aceh Province, Indonesia.
A total of 100 fecal samples were collected from wild macaques at four sites: Pulau Weh Sabang Nature Tourism Park, Kuala Langsa Mangrove Forest, Saree (Aceh Besar), and Aceh Jaya. The parasitological examination involved the centrifugation method, lactophenol staining, and scanning electron microscopy (SEM). Parasites were identified based on egg morphology and adult worm anatomy.
Of the 100 samples analyzed, 45% tested positive for GI parasites. Nematode prevalence was highest (80%), followed by protozoa (10%) and ectoparasites (10%). Identified nematodes included spp. (70%), spp. (50%), spp. (40%), spp. (30%), spp. (20%), and spp. (10%). Protozoan ( spp.) and ectoparasitic ( spp.) infections were less common. Mixed infections were more frequent (70%) than single infections (30%). Adult worms examined through SEM and lactophenol staining were confirmed to be two nematode species: () and , both with zoonotic potential.
The high prevalence of nematodes, particularly zoonotic species, underscores the importance of monitoring parasitic infections in residing in tourist-exposed areas. This study highlights the utility of combining traditional and advanced diagnostic techniques to enhance parasite surveillance. Integrating health assessments of wild primate populations into conservation programs is recommended to mitigate zoonotic risk and support One Health objectives.
由于食蟹猴在生态上与人类种群接近，它们是动物源性疾病监测的关键哨兵。了解它们的寄生虫负担对于保护和公共卫生至关重要，特别是在人类与灵长类动物互动频繁的生态旅游地区。本研究旨在调查印度尼西亚亚齐省四个自然栖息地的食蟹猴胃肠道（GI）寄生虫和体表寄生虫的流行情况、多样性及形态特征。
在四个地点从野生猕猴收集了总共100份粪便样本：韦岛沙璜自然旅游公园、瓜拉兰萨红树林、萨雷（大亚齐）和亚齐贾亚。寄生虫学检查采用离心法、乳酸酚染色和扫描电子显微镜（SEM）。根据虫卵形态和成虫解剖结构鉴定寄生虫。
在分析的100个样本中，45%的样本检测出胃肠道寄生虫呈阳性。线虫的流行率最高（80%），其次是原生动物（10%）和体表寄生虫（10%）。鉴定出的线虫包括 属（70%）、 属（50%）、 属（40%）、 属（30%）、 属（20%）和 属（10%）。原生动物（ 属）和体表寄生虫（ 属）感染较少见。混合感染比单一感染更频繁（70%）。通过扫描电子显微镜和乳酸酚染色检查的成虫被确认为两种线虫： （ ） 和 ，两者都具有人畜共患病潜力。
线虫的高流行率，特别是具有人畜共患病潜力的物种，凸显了监测生活在旅游暴露地区的食蟹猴寄生虫感染的重要性。本研究强调了结合传统和先进诊断技术以加强寄生虫监测的实用性。建议将野生灵长类动物种群的健康评估纳入保护计划，以减轻人畜共患病风险并支持“同一健康”目标。
