恒牙和乳牙牙髓干细胞的分离与培养。
Isolation and culture of dental pulp stem cells from permanent and deciduous teeth.
作者信息
Naz Shagufta, Khan Farhan Raza, Zohra Raheela Rahmat, Lakhundi Sahreena Salim, Khan Mehwish Sagheer, Mohammed Nuruddin, Ahmad Tashfeen
机构信息
Ms. Shagufta Naz, M.Sc., Department of Surgery, Department of Biotechnology, University of Karachi, Pakistan. Aga Khan University, Karachi, Pakistan.
Dr. Farhan Raza Khan, FCPS, Department of Surgery, Aga Khan University, Karachi, Pakistan.
出版信息
Pak J Med Sci. 2019 Jul-Aug;35(4):997-1002. doi: 10.12669/pjms.35.4.540.
OBJECTIVE
To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth.
METHODS
It was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics.
RESULTS
As above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while permanent teeth pulp took 12.4±3.7 days. Together, 26.6±3.6 and 24.5±3.5 days were required for permanent and deciduous tooth pulp stem cells to be ready for further assays.
CONCLUSIONS
The protocol we developed is easy and consistent and can be used to generate reliable source of MScs for engineering of calcified and non-calcified tissue for regenerative medicine approaches.
目的
从未感染的人类恒牙和乳牙中分离牙髓间充质干细胞（MSCs）。
方法
这是一项体外实验研究。从13名表面健康的受试者（包括9名成年人和4名儿童）收集人类牙齿。去冠后，从牙齿中取出牙髓，并通过外植体法在干细胞专用培养基中培养。数据采用描述性统计进行分析。
结果
如上所述，MSCs出现，呈现成纤维细胞样形态。体外培养中，恒牙和乳牙的阳性率分别为100%（9/9）和75%（3/4）。乳牙牙髓中第一个细胞在10±6.2天出现，而恒牙牙髓则需要12.4±3.7天。恒牙和乳牙牙髓干细胞准备好进行进一步检测分别需要26.6±3.6天和24.5±3.5天。
结论
我们开发的方案简便且一致，可用于为再生医学方法中钙化和非钙化组织工程生成可靠的MSCs来源。
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