Koul Jharna, Agnihotri Divya, Jhambh Ricky, Kumar Tarun, Charaya Gaurav
Department of Veterinary Medicine, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
Department of Veterinary Clinical Complex, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
Vet Res Forum. 2025;16(5):261-265. doi: 10.30466/vrf.2024.2030571.4301. Epub 2025 May 15.
The present study was planned to confirm the clinical diagnosis of canine distemper in dogs with reverse transcriptase polymerase chain reaction (RT-PCR) and its comparison with lateral flow assay based immuno-chromatographic (IC) technique. Fifty clients owned dogs having clinical signs suggestive of respiratory, skin or nervous form of canine distemper were included in the study. An immuno-chromatography-based test was applied using serum to screen each of the suspected dog. In addition to serum, nasal discharges of 15 cases and ocular discharges of 10 samples were used to diagnose canine distemper. Screening with IC kit revealed 72.00% serum samples positive, 66.66% ocular and 50.00% nasal samples were found positive for antigen. The RT-PCR targeting N gene of canine distemper virus was used for the molecular diagnosis of canine distemper. Out of 50 blood samples tested with RT-PCR assay, 38 (76.00%) samples were positive showing characteristics band of 287bp. Statistical comparison of IC Kit (serum samples) results with RT-PCR results comparison showed that IC kit was 52.00% accurate with 36.84% sensitivity, 100% specificity, positive predictive value of 93.33% and negative predictive value of 31.43%. In the current study it was observed that the IC test was rapid, quick and specific but was found to be less sensitive compared to RT-PCR.
本研究旨在通过逆转录聚合酶链反应（RT-PCR）来确诊犬瘟热，并将其与基于免疫层析（IC）技术的侧向流动分析进行比较。50位饲养有疑似患有呼吸道、皮肤或神经型犬瘟热临床症状犬只的客户被纳入研究。使用血清进行基于免疫层析的检测，以筛查每只疑似犬。除血清外，还使用了15例的鼻分泌物和10份眼分泌物来诊断犬瘟热。用IC试剂盒筛查显示，72.00%的血清样本呈阳性，66.66%的眼分泌物样本及50.00%的鼻分泌物样本抗原呈阳性。针对犬瘟热病毒N基因的RT-PCR用于犬瘟热的分子诊断。在50份用RT-PCR检测的血液样本中，38份（76.00%）样本呈阳性，显示出287bp的特征条带。IC试剂盒（血清样本）结果与RT-PCR结果的统计比较显示，IC试剂盒的准确率为52.00%，灵敏度为36.84%，特异性为100%，阳性预测值为93.33%，阴性预测值为31.43%。在本研究中观察到，IC检测快速、简便且具有特异性，但与RT-PCR相比，其灵敏度较低。
