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罗尔夫的分离与基因组注释,一种具有紫色酸性磷酸酶样基因的新型簇状EG噬菌体。

Isolation and genome annotation of Rowlf, a novel cluster EG bacteriophage with a purple acid phosphatase-like gene.

作者信息

Sullivan Emily, Mireill Ninahazwe, Allen Aisley B, Larsen Phoenix R, Puentes Navarro Daniel, Renn Madison P, Shappee Trenton W, Sisk Madalyne M, Thomas Alexander W, Trejo Giselle, Bellew Lexy, George Lee, Hepworth Sariah, Webb Savannah, Sadeghi Sara, Burger Joshua, Githuku Eva, Shurley Jack F, Grinath Anna S, Thomas Michael A

机构信息

Department of Biological Sciences, Idaho State University, Pocatello, Idaho, USA.

出版信息

Microbiol Resour Announc. 2025 Aug 14;14(8):e0031425. doi: 10.1128/mra.00314-25. Epub 2025 Jul 22.

Abstract

Isolated from grass thatch in Pocatello, Idaho, phage Rowlf has a siphovirus morphology and is presumed to be a lytic bacteriophage. Rowlf has a 62,044 bp genome with 104 putative genes, including a purple acid phosphatase-like gene found in leguminous plants and bacteria. Based on similarity to actinobacteriophages, Rowlf was assigned cluster EG.

摘要

噬菌体罗尔夫是从爱达荷州波卡特洛的茅草中分离出来的,具有丝杆病毒形态,被认为是一种裂解性噬菌体。罗尔夫有一个62044碱基对的基因组,包含104个推定基因,其中包括一个在豆科植物和细菌中发现的类紫色酸性磷酸酶基因。基于与放线菌噬菌体的相似性,罗尔夫被归入EG簇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/074f/12352085/65dfa5623a7a/mra.00314-25.f001.jpg

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