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罗尔夫的分离与基因组注释,一种具有紫色酸性磷酸酶样基因的新型簇状EG噬菌体。

Isolation and genome annotation of Rowlf, a novel cluster EG bacteriophage with a purple acid phosphatase-like gene.

作者信息

Sullivan Emily, Mireill Ninahazwe, Allen Aisley B, Larsen Phoenix R, Puentes Navarro Daniel, Renn Madison P, Shappee Trenton W, Sisk Madalyne M, Thomas Alexander W, Trejo Giselle, Bellew Lexy, George Lee, Hepworth Sariah, Webb Savannah, Sadeghi Sara, Burger Joshua, Githuku Eva, Shurley Jack F, Grinath Anna S, Thomas Michael A

机构信息

Department of Biological Sciences, Idaho State University, Pocatello, Idaho, USA.

出版信息

Microbiol Resour Announc. 2025 Aug 14;14(8):e0031425. doi: 10.1128/mra.00314-25. Epub 2025 Jul 22.

DOI:10.1128/mra.00314-25
PMID:40693759
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12352085/
Abstract

Isolated from grass thatch in Pocatello, Idaho, phage Rowlf has a siphovirus morphology and is presumed to be a lytic bacteriophage. Rowlf has a 62,044 bp genome with 104 putative genes, including a purple acid phosphatase-like gene found in leguminous plants and bacteria. Based on similarity to actinobacteriophages, Rowlf was assigned cluster EG.

摘要

噬菌体罗尔夫是从爱达荷州波卡特洛的茅草中分离出来的,具有丝杆病毒形态,被认为是一种裂解性噬菌体。罗尔夫有一个62044碱基对的基因组,包含104个推定基因,其中包括一个在豆科植物和细菌中发现的类紫色酸性磷酸酶基因。基于与放线菌噬菌体的相似性,罗尔夫被归入EG簇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/074f/12352085/65dfa5623a7a/mra.00314-25.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/074f/12352085/65dfa5623a7a/mra.00314-25.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/074f/12352085/65dfa5623a7a/mra.00314-25.f001.jpg

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本文引用的文献

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Isolation of Bacteriophages on Actinobacteria Hosts.放线菌宿主噬菌体的分离。
Methods Mol Biol. 2024;2793:273-298. doi: 10.1007/978-1-0716-3798-2_17.
2
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Microbiol Resour Announc. 2023 Dec 14;12(12):e0094323. doi: 10.1128/MRA.00943-23. Epub 2023 Nov 22.
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Application of bacteriophage cocktails for reducing the bacterial load of nosocomial pathogens in hospital wastewater.
噬菌体鸡尾酒疗法在降低医院废水中医院病原体细菌载量方面的应用。
Iran J Microbiol. 2022 Jun;14(3):395-401. doi: 10.18502/ijm.v14i3.9782.
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Isolation of new phage PSKP16.新型噬菌体PSKP16的分离
Iran J Microbiol. 2023 Feb;15(1):62-68. doi: 10.18502/ijm.v15i1.11919.
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Annotation of the Complete Genome Sequences of Bacteriophages Sara and Birdfeeder.噬菌体萨拉和喂鸟器的全基因组序列注释
Microbiol Resour Announc. 2022 Oct 20;11(10):e0078022. doi: 10.1128/mra.00780-22. Epub 2022 Sep 19.
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Phage Therapy in the Era of Multidrug Resistance in Bacteria: A Systematic Review.噬菌体疗法在细菌多重耐药时代:系统评价。
Int J Mol Sci. 2022 Apr 21;23(9):4577. doi: 10.3390/ijms23094577.
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Complete Genome Sequence of Microbacterium foliorum NRRL B-24224, a Host for Bacteriophage Discovery.叶状微杆菌NRRL B-24224的全基因组序列,一种用于噬菌体发现的宿主菌
Microbiol Resour Announc. 2019 Jan 31;8(5). doi: 10.1128/MRA.01467-18. eCollection 2019 Jan.
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Methods Mol Biol. 2018;1681:109-125. doi: 10.1007/978-1-4939-7343-9_9.
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Sci Rep. 2017 Sep 8;7(1):11012. doi: 10.1038/s41598-017-11490-9.
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Bacteriophages of spp. Display a Spectrum of Diversity and Genetic Relationships.[细菌名称]属的噬菌体表现出多样的谱系和遗传关系。 (注:原文中“spp.”指代不明,这里用[细菌名称]属来表示需根据实际指代细菌来准确翻译)
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