Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA, USA.
Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Methods Mol Biol. 2024;2793:273-298. doi: 10.1007/978-1-0716-3798-2_17.
Bacteriophages are ubiquitous biological entities which can be found in a variety of habitats. Here, we describe protocols for the isolation of bacteriophages on a variety of Actinobacterial genera. Two approaches to phage isolation, direct isolation and enriched isolation, are described, which can be performed individually or in parallel. The protocols described can be adapted to isolate a wide array of bacteriophages.
噬菌体是无处不在的生物实体，可以在各种生境中找到。在这里，我们描述了从各种放线菌属中分离噬菌体的方案。描述了两种噬菌体分离方法，直接分离法和富集分离法，可以单独或并行进行。所描述的方案可以适应广泛的噬菌体的分离。
