新型噬菌体PSKP16的分离
Isolation of new phage PSKP16.
作者信息
Sadeqi Sara, Michniewski Slawomir, Nikkhahi Farhad, Jameson Eleanor, Marashi Seyed Mahmoud Amin
机构信息
Department of Medical Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Life Sciences, University of Warwick, Coventry, United Kingdom.
出版信息
Iran J Microbiol. 2023 Feb;15(1):62-68. doi: 10.18502/ijm.v15i1.11919.
BACKGROUND AND OBJECTIVES
is a clinically relevant opportunistic pathogen belonging to the family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.
MATERIALS AND METHODS
Phage PSKP16 was isolated against , capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a -like morphology.
RESULTS
PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus and shows high evolutionary proximity to phages JY917, Sushi, and B1.
CONCLUSION
Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.
背景与目的
是一种属于该菌属的具有临床相关性的机会致病菌。它是全球与抗菌药物耐药性死亡相关的三大细菌之一，也是引起医院感染的最危险细菌之一。噬菌体疗法为治疗耐药细菌感染提供了一种潜在选择。
材料与方法
分离出针对K2型荚膜（从伤口感染中分离）的噬菌体PSKP16。PSKP16是一种具有样形态的新型裂解性噬菌体。
结果
PSKP16是一种线性双链DNA噬菌体，GC含量为50%，基因组大小为46712 bp，预测有67个开放阅读框。PSKP16属于该属，与噬菌体JY917、Sushi和B1具有高度进化亲缘关系。
结论
噬菌体分离快速、廉价且高效，但需要时间和鉴定（这会增加成本）以确保分离出的噬菌体不会对健康构成风险，这对于安全使用噬菌体疗法治疗危及生命的细菌感染至关重要。
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