Şenocak Ahmet, Kızılkurtlu Ahmet Akif, Sanko Vildan, Tümay Süreyya Oğuz, Demirbaş Ümit
Department of Chemistry, Gebze Technical University, Gebze/Kocaeli 41400, Türkiye.
Biotechnology Institute, Gebze Technical University, Gebze/Kocaeli 41400, Türkiye.
Langmuir. 2025 Aug 5;41(30):20297-20307. doi: 10.1021/acs.langmuir.5c02632. Epub 2025 Jul 22.
Prostate-specific antigen (PSA) is a protein produced by the prostate gland and is a critical biomarker for the early detection and monitoring of prostate cancer. Current methods in the detection of PSA include enzyme-linked immunosorbent assay (ELISA), chemiluminescent immunoassays (CLIA), and radioimmunoassay (RIA). They are expensive and time-consuming processes, even though they are considered the gold standard. On the other hand, biosensing technology is an emerging alternative method for the detection of PSA. Electrochemical immunosensor systems are widely used for detecting a variety of antigens with high selectivity and specificity. In this study, to prepare an immunosensor for PSA detection, a thiophene appended cobalt(II) phthalocyanine (CoPc), its polymer (Poly-Pc), and CuCoFeO@MoS-NH were synthesized. Then, anti-PSA antibodies were immobilized on the CuCoFeO@MoS-NH-based Poly-Pc composite using glutaraldehyde as a cross-linker, and the immunosensor was prepared. Differential pulse voltammetry (DPV) employed to monitor the measurements showed that the proposed immunosensor system exhibited excellent analytical performance, with a wide linear detection range (0.01-1000 ng/mL) and a low detection limit of 6.3 pg/mL. The proposed immunosensor system offered high selectivity, reproducibility, and stability, even in complex biological samples such as synthetic serum, which was compared to a commercially available ELISA kit. The obtained results showed that the immunosensor has the potential to be used in clinical diagnosis as a rapid and reliable tool for PSA detection.
前列腺特异性抗原（PSA）是一种由前列腺分泌的蛋白质，是早期检测和监测前列腺癌的关键生物标志物。目前检测PSA的方法包括酶联免疫吸附测定（ELISA）、化学发光免疫测定（CLIA）和放射免疫测定（RIA）。尽管这些方法被视为金标准，但它们成本高昂且耗时。另一方面，生物传感技术是一种新兴的PSA检测替代方法。电化学免疫传感器系统被广泛用于以高选择性和特异性检测各种抗原。在本研究中，为制备用于检测PSA的免疫传感器，合成了噻吩取代的钴（II）酞菁（CoPc）、其聚合物（聚酞菁）以及CuCoFeO@MoS-NH。然后，使用戊二醛作为交联剂将抗PSA抗体固定在基于CuCoFeO@MoS-NH的聚酞菁复合材料上，制备了免疫传感器。采用差分脉冲伏安法（DPV）监测测量结果，结果表明所提出的免疫传感器系统具有出色的分析性能，线性检测范围宽（0.01 - 1000 ng/mL），检测限低至6.3 pg/mL。与市售ELISA试剂盒相比，所提出的免疫传感器系统即使在合成血清等复杂生物样品中也具有高选择性、重现性和稳定性。所得结果表明，该免疫传感器有潜力作为一种快速可靠的PSA检测工具用于临床诊断。
