Saraban Muktinan, Khumngern Suntisak, Nontipichet Natha, Kangkamano Tawatchai, Lomae Atchara, Thavarungkul Panote, Bejrananda Tanan, Numnuam Apon
Center of Excellence for Trace Analysis and Biosensor, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Center of Excellence Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Center of Excellence for Trace Analysis and Biosensor, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Talanta. 2025 Aug 2;297(Pt A):128669. doi: 10.1016/j.talanta.2025.128669.
A novel label-free electrochemical immunosensor was constructed to quantify prostate-specific antigen (PSA) in human serum. A poly(3,4-ethylenedioxythiophene) and Prussian blue nanocomposite (PEDOT@PB) was used to a screen-printed carbon electrode (SPCE) modification to construct the platform followed by a nanocomposite of reduced graphene oxide and copper metal-organic framework (rGO@Cu(BDC-NH) MOF), that was then decorated with porous platinum nanoparticles (PPtNPs). In this system, PEDOT@PB served as a redox probe. The rGO@Cu(BDC-NH) MOF offered a high surface area and exceptional electroconductivity to facilitate PPtNPs chemisorption through coordination with the amino groups of Cu(BDC-NH) MOF. The PPtNPs provided high conductivity and a high surface area to enhance the immobilization of PSA antibody (Ab), thereby improving sensor performance. PSA was quantified using differential pulse voltammetry (DPV) by monitoring the decrease in the oxidation peak current of the PB probe as the antigen-antibody immunocomplex forms to create an insulating layer that hinders electron transfer. The proposed sensor demonstrated excellent performances under optimized conditions, with a linear range of 1.0 × 10 to 10 ng mL and a low limit of detection of 7.8 × 10 ng mL. The sensor also demonstrated excellent stability, enhanced sensitivity, and acceptable selectivity. There was no meaningful statistical difference between the results obtained from the sensor and those from the electrochemiluminescence immunoassay (P > 0.05).
构建了一种新型无标记电化学免疫传感器，用于定量检测人血清中的前列腺特异性抗原（PSA）。使用聚（3,4-乙撑二氧噻吩）和普鲁士蓝纳米复合材料（PEDOT@PB）对丝网印刷碳电极（SPCE）进行修饰以构建平台，随后是还原氧化石墨烯和铜金属有机框架（rGO@Cu(BDC-NH) MOF）的纳米复合材料，然后用多孔铂纳米颗粒（PPtNPs）进行修饰。在该系统中，PEDOT@PB用作氧化还原探针。rGO@Cu(BDC-NH) MOF提供了高表面积和出色的导电性，通过与Cu(BDC-NH) MOF的氨基配位促进PPtNPs的化学吸附。PPtNPs提供了高导电性和高表面积，以增强PSA抗体（Ab）的固定，从而提高传感器性能。通过监测PB探针氧化峰电流的下降来使用差分脉冲伏安法（DPV）对PSA进行定量，因为抗原-抗体免疫复合物形成了阻碍电子转移的绝缘层。所提出的传感器在优化条件下表现出优异的性能，线性范围为1.0×10至10 ng mL，检测下限低至7.8×10 ng mL。该传感器还表现出出色的稳定性、增强的灵敏度和可接受的选择性。传感器获得的结果与电化学发光免疫分析获得的结果之间没有显著的统计学差异（P>0.05）。
