Sorahinobar Mona, Yusefieh Nastaran, Rezayian Maryam, Shahbazi Samira
Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Department of Plant Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Sci Rep. 2025 Jul 22;15(1):26552. doi: 10.1038/s41598-025-08922-2.
This study investigated the effects of seed biopriming with two Trichoderma harzianum isolates, wild type (TW) and gamma-ray mutant (TM), on drought tolerance in the two barley cultivars, Goharan and Nosrat. Barley seeds primed with Trichoderma and subsequently exposed to drought stress induced by polyethylene glycol (PEG 6000) at concentrations of 0%, 5% and 10%. Drought stress significantly reduced growth (20-45% decrease in fresh and dry weight), with Nosrat showing a greater reduction than Goharan. Biopriming with TW and TM significantly improved plant growth (fresh and dry biomass by 4-56%), particularly with TM, and alleviated drought-induced oxidative stress (reduced HO and MDA levels) by enhancing antioxidant defense systems (increased superoxide dismutase, peroxidase, and polyphenol oxidase activity) and non-enzymatic antioxidant content (total phenolics, flavonoids, and anthocyanins). TW and TM priming also improved osmotic regulation (increased protein and soluble sugars) and increased IAA content and the activity of key respiratory enzymes (aconitase, succinate dehydrogenase, malate dehydrogenase, and fumarase). These findings highlight the multifaceted role of biopriming with T. harzianum isolates in enhancing adaptive responses of barley to drought stress. Seed biopriming with TW and TM significantly improved barley cultivar resilience under drought conditions, suggesting their potential as effective drought tolerance strategies for crops.
本研究调查了用两种哈茨木霉菌株，即野生型（TW）和γ射线突变型（TM）对大麦种子进行生物引发处理，对两个大麦品种戈哈兰和诺斯拉特耐旱性的影响。用木霉菌引发处理的大麦种子，随后在浓度为0%、5%和10%的聚乙二醇（PEG 6000）诱导的干旱胁迫下处理。干旱胁迫显著降低了生长量（鲜重和干重下降20 - 45%），诺斯拉特的下降幅度比戈哈兰更大。用TW和TM进行生物引发处理显著改善了植株生长（鲜生物量和干生物量增加4 - 56%），特别是TM处理，通过增强抗氧化防御系统（超氧化物歧化酶、过氧化物酶和多酚氧化酶活性增加）和非酶抗氧化剂含量（总酚、类黄酮和花青素）减轻了干旱诱导的氧化胁迫（降低了过氧化氢和丙二醛水平）。TW和TM引发处理还改善了渗透调节（蛋白质和可溶性糖增加），增加了生长素含量以及关键呼吸酶（乌头酸酶、琥珀酸脱氢酶、苹果酸脱氢酶和延胡索酸酶）的活性。这些发现突出了用哈茨木霉菌株进行生物引发处理在增强大麦对干旱胁迫适应性反应中的多方面作用。用TW和TM对种子进行生物引发处理显著提高了干旱条件下大麦品种的恢复力，表明它们作为作物有效耐旱策略的潜力。
