Kamani Joshua, Irene Sacristán, Yakubu Arin R, Bwala Falmata H, Nahum-Biala Yaarit, Nnabuife Ekene H, Budaye James, Harrus Shimon, Schaer Juliane
National Veterinary Research Institute (NVRI) Vom Plateau Nigeria.
Animal Health Research Centre National Institute for Agricultural and Food Research and Technology (INIA) Centro Superior de Investigaciones Científicas (CSIC) Valdeolmos Madrid Spain.
Public Health Chall. 2025 Jul 22;4(3):e70079. doi: 10.1002/puh2.70079. eCollection 2025 Sep.
Malaria remains a significant threat in high-burden high-impact (HBHI) countries despite substantial investments in disease control. This highlights the need for more comprehensive and inclusive strategies to meet national and international targets. Although agricultural and poorly maintained environments are known for mosquito breeding, workplaces are rarely considered in conventional malaria control measures. In this pilot investigation, we assessed the presence of spp. in a laboratory-office complex in Vom, Nigeria, to assess workplace malaria risk and its implications for control strategies. We conducted molecular barcoding on 74 specimens targeting the mitochondrial cytochrome oxidase I gene (). Our analyses identified ( = 29; 54.6%), sensu lato ( = 17; 32.1%), and ( = 6; 11.3%). Haplotype network analyses revealed 12, 8, and 6 distinct haplotypes for . , . , and . , respectively. Genetic divergence estimates for sequences were ≤0.011% for , ≤0.007% for , and ≤0.018% for . The detection of genetically diverse vector species in an office setting underscores the potential risk of workplace malaria transmission. This pilot study provides initial evidence that workplace environments can harbor genetically diverse malaria vectors and should be considered in future surveillance and control strategies. We recommend subnational tailoring (SNT) of intervention strategies to incorporate workplace environments and public places into malaria control efforts.
尽管在疾病控制方面投入了大量资金，但疟疾在高负担高影响（HBHI）国家仍然构成重大威胁。这凸显了需要更全面和包容性更强的战略来实现国家和国际目标。虽然农业环境和维护不善的环境以蚊虫滋生而闻名，但传统的疟疾控制措施很少考虑工作场所。在这项试点调查中，我们评估了尼日利亚 Vom 的一个实验室 - 办公室综合区域中疟蚊属物种的存在情况，以评估工作场所的疟疾风险及其对控制策略的影响。我们针对线粒体细胞色素氧化酶 I 基因（ ）对 74 个蚊虫样本进行了分子条形码分析。我们的分析确定了冈比亚按蚊（ = 29；54.6%）、广义嗜人按蚊（ = 17；32.1%）和阿拉伯按蚊（ = 6；11.3%）。单倍型网络分析分别揭示了冈比亚按蚊、广义嗜人按蚊和阿拉伯按蚊的 12、8 和 6 种不同单倍型。冈比亚按蚊序列的遗传分歧估计值 ≤0.011%，广义嗜人按蚊 ≤0.007%，阿拉伯按蚊 ≤0.018%。在办公环境中检测到基因多样的疟蚊媒介物种凸显了工作场所疟疾传播的潜在风险。这项试点研究提供了初步证据，表明工作场所环境可能存在基因多样的疟疾媒介，未来的监测和控制策略应予以考虑。我们建议对干预策略进行次国家级调整（SNT），将工作场所环境和公共场所纳入疟疾控制工作中。
