Guo Xiao, Xing Jiaying, Shi Xinlin, Cao Yuyan, An Liping
School of Pharmacy, Beihua University, Jilin, Jilin, 132000, P. R. China.
Naturwissenschaften. 2025 Jul 23;112(4):53. doi: 10.1007/s00114-025-02002-1.
The objective of the study is to establish an extraction method of exosomes (Exos) from neural stem cells (NSCs) and to explore the effect of exosomes on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, MPTP). In this study, neural stem cell-derived exosomes (NSC-Exos) were extracted for treatment; a mouse model of PD induced by MPTP was established. After the treatment with NSC-Exos, the behavioral ability of mice in the EXO group was significantly improved compared to that in the MPTP group. The biochemical index test results showed that compared with that in the MPTP group, the activity of SOD and the content of GSH in the serum of mice in the EXO group increased (P < 0.01), while the content of MDA decreased (P < 0.05). Hematoxylin-eosin staining (HE) was used to observe the histopathological morphological changes in the brain of PD mice. Western blot results showed that compared with that in the MPTP group, after the treatment with NSC-Exos, the expression level of α-syn protein in the brain tissue of mice in the EXO group decreased, while that of TH protein increased (P < 0.05); compared with those in the MPTP group, the expression levels of I-κB, NF-κB, IL-6, IL-1β, and TNF-α proteins increased (P < 0.01). The 16S rDNA sequencing results of mouse gut microbiota showed that compared with that in the MPTP group, the abundance of Firmicutes decreased (P < 0.05), while the abundance of Bacteroidetes and Actinobacteria increased (P < 0.05) in the EXO group. The results of this study indicate that NSC-Exos have a certain therapeutic effect on the MPTP-induced PD in mice.
本研究的目的是建立从神经干细胞（NSCs）中提取外泌体（Exos）的方法，并探讨外泌体对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）的影响。在本研究中，提取神经干细胞来源的外泌体（NSC-Exos）进行治疗；建立MPTP诱导的帕金森病（PD）小鼠模型。用NSC-Exos治疗后，EXO组小鼠的行为能力较MPTP组有显著改善。生化指标检测结果显示，与MPTP组相比，EXO组小鼠血清中SOD活性和GSH含量升高（P＜0.01），而MDA含量降低（P＜0.05）。采用苏木精-伊红染色（HE）观察PD小鼠脑内组织病理学形态变化。蛋白质免疫印迹结果显示，与MPTP组相比，NSC-Exos治疗后，EXO组小鼠脑组织中α-突触核蛋白表达水平降低，而TH蛋白表达水平升高（P＜0.05）；与MPTP组相比，I-κB、NF-κB、IL-6、IL-1β和TNF-α蛋白表达水平升高（P＜0.01）。小鼠肠道微生物群的16S rDNA测序结果显示，与MPTP组相比，EXO组小鼠肠道中厚壁菌门丰度降低（P＜0.05），而拟杆菌门和放线菌门丰度升高（P＜0.05）。本研究结果表明，NSC-Exos对MPTP诱导的小鼠PD具有一定的治疗作用。
