Connolly Mona, Flahaut Emmanuel, Navas José María
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC), Carretera de la Coruña Km 7,5, E-28040 Madrid, Spain.
Centre National de la Recherche Scientifique (CNRS), Toulouse INP, Centre Interuniversitaire de Recherche et d'Ingénierie des MATériaux (CIRIMAT), Université de Toulouse, 118 Route de Narbonne, 31062 Toulouse Cedex 9, France.
J Xenobiot. 2025 Jun 24;15(4):97. doi: 10.3390/jox15040097.
Hexagonal boron nitride (h-BN) and boron nitride nanotubes (BNNTs) are emerging advanced nanomaterials with analogous structures to graphene and carbon nanotubes, respectively. However, little is known about what effect replacing carbon atoms with boron and nitrogen will have on the materials' safety profile. This study's aim was to first identify if multi-walled nanotubes of BN could produce a hazard profile similar to that evidenced already for multi-walled carbon nanotubes (MWCNTs) and secondly if the material when present in a sheet-like structure increases or decreases the hazard profile. Fish are aquatic organisms sensitive to boron compounds; however, the potential hazard following exposure to BN and especially when present in such nanostructures has not yet been investigated. An in vitro testing platform consisting of multiple cell lines of the rainbow trout, (RTH-149, RTG-2, RTL-W1 and RTgill-W1), was used in a first-hazard screening approach for cytotoxicity and to gain information on material-cellular interaction. Clear differences were evidenced in material uptake, leading to plasma membrane disruption accompanied with a loss in metabolic activity for BNNTs at lower exposure concentrations compared to h-BN. As in the case of carbon nanotubes, close attention must be given to potential interferences with assays based on optical readouts.
六方氮化硼（h-BN）和氮化硼纳米管（BNNTs）是新兴的先进纳米材料，其结构分别与石墨烯和碳纳米管相似。然而，用硼和氮取代碳原子会对材料的安全性产生何种影响，目前所知甚少。本研究的目的首先是确定BN多壁纳米管是否会产生与多壁碳纳米管（MWCNTs）已证实的类似危害特征，其次是确定呈片状结构的该材料会增加还是降低危害特征。鱼类是对硼化合物敏感的水生生物；然而，接触BN尤其是接触此类纳米结构时的潜在危害尚未得到研究。在首次危害筛选中，使用了由虹鳟鱼的多种细胞系（RTH-149、RTG-2、RTL-W1和RTgill-W1）组成的体外测试平台，以检测细胞毒性并获取有关材料与细胞相互作用的信息。在材料摄取方面存在明显差异，与h-BN相比，较低暴露浓度下的BNNTs会导致质膜破坏并伴有代谢活性丧失。与碳纳米管的情况一样，必须密切关注基于光学读数的检测可能受到的干扰。
