The motor response to human visual stimuli is unique and differs from the reaction to light-based visual stimuli. While laboratory-based tests offer valuable insights into athletes' basic perceptual-motor abilities, their translation to actual sports-specific tests is limited. Methods: Following a thorough warm-up, 44 collegiate-level male soccer players (age: 24.4 ± 2.5 y, mass: 63.01 ± 7.3 kg, stature: 167.62 ± 6.3 cm) from a tertiary institution completed the following tests: Sports Vision Test (20-light proactive speed test), 40 m sprint test (split times over 5, 10, 20 and 40 m), and a live Reactive Agility Test (RAT) entailing them to sprint, change direction either towards their dominant limb or non-dominant limb in response to a live tester, and sprint again. : Numerous moderate correlations were seen between the RAT and various sprint distances (r > 0.3, ES > 0.3, < 0.05). The reaction speed relationship between the light-based (SVT) and live stimuli (RAT) test yielded a weak relationship (r > 0.4, ES > 0.5, < 0.05). Furthermore, the light-based hand-eye coordination speed did not predict acceleration or top speed, while the total RAT time did explain 10.5% of top speed (40 m). No significant differences in the SVT average and total time were found among playing positions. : The limited correlations observed indicate that light-based reaction training alone may not be sufficient to translate to field-based reactive agility; therefore, training should integrate perceptual-cognitive and motor demands. Future research should refine laboratory-based tests by incorporating contextual elements to enhance ecological validity and further investigate the transferability of these skills from controlled settings to real-world game scenarios.