Ungur Andreea Petra, Socaciu Andreea-Iulia, Barsan Maria, Rajnoveanu Armand Gabriel, Ionut Razvan, Socaciu Carmen, Procopciuc Lucia Maria
Department of Occupational Health, University of Medicine and Pharmacy "Iuliu Haţieganu", Str. Victor Babes 8, 400347 Cluj-Napoca, Romania.
Research Center for Applied Biotechnology and Molecular Therapy Biodiatech, SC Proplanta Str. Trifoiului 12G, 400478 Cluj-Napoca, Romania.
Clocks Sleep. 2025 Jul 14;7(3):36. doi: 10.3390/clockssleep7030036.
Burnout syndrome is characterized mainly by three criteria (emotional exhaustion, depersonalization, and low personal accomplishment), and further exacerbated by night shift work, with profound implications for individual and societal well-being. The Maslach Burnout Inventory survey applied to 97 medical care professionals (with day and night work) revealed different scores for these criteria. Blood metabolic profiles were obtained by UHPLC-QTOF-ESI-MS untargeted metabolomics and multivariate statistics using the Metaboanalyst 6.0 platform. The Partial Least Squares Discrimination scores and VIP values, Random Forest graphs, and Heatmaps, based on 99 identified metabolites, were complemented with Biomarker Analysis (AUC ranking) and Pathway Analysis of metabolic networks. The data obtained reflected the biochemical implications of night shift work and correlated with each criterion's burnout scores. Four main metabolic pathways with important consequences in burnout were affected, namely lipid metabolism, especially steroid hormone synthesis and cortisol, the energetic mitochondrial metabolism involving acylated carnitines, fatty acids, and phospholipids as well polar metabolites' metabolism, e.g., catecholamines (noradrenaline, acetyl serotonin), and some amino acids (tryptophan, tyrosine, aspartate, arginine, valine, lysine). These metabolic profiles suggest potential strategies for managing burnout levels in healthcare professionals, based on validated criteria, including night shift work management.
职业倦怠综合征主要由三个标准（情感耗竭、去人格化和低个人成就感）来表征，并因夜班工作而进一步加剧，这对个人和社会福祉有着深远影响。对97名医疗保健专业人员（包括日班和夜班工作者）进行的马氏职业倦怠量表调查显示，这些标准的得分有所不同。通过超高效液相色谱-四极杆飞行时间-电喷雾电离质谱非靶向代谢组学方法，并使用Metaboanalyst 6.0平台进行多元统计分析，获得了血液代谢谱。基于99种已鉴定的代谢物，偏最小二乘判别得分和VIP值、随机森林图以及热图，通过生物标志物分析（AUC排名）和代谢网络通路分析得到了补充。所获得的数据反映了夜班工作的生化影响，并与每个标准的职业倦怠得分相关。有四个在职业倦怠中产生重要影响的主要代谢途径受到了影响，即脂质代谢，特别是类固醇激素合成和皮质醇，涉及酰化肉碱、脂肪酸和磷脂的线粒体能量代谢以及极性代谢物的代谢，例如儿茶酚胺（去甲肾上腺素、乙酰血清素）和一些氨基酸（色氨酸、酪氨酸、天冬氨酸、精氨酸、缬氨酸、赖氨酸）。这些代谢谱基于已验证的标准，包括夜班工作管理，为管理医疗保健专业人员的职业倦怠水平提供了潜在策略。
