Malaveille C, Planche G, Bartsch H
Chem Biol Interact. 1977 May;17(2):129-36. doi: 10.1016/0009-2797(77)90079-5.
Factors were studied which modify the enzymatic capacity of mouse liver microsomal mixed-function oxidase to convert vinylidene chloride (1.1-dichloroethylene) (VDC) into mutagens in the Salmonella/microsome mutagenicity test. A microsomal fraction incorporated in soft agar layer converted VDC into mutagens during 7 h at a constant rate; these were detected with S. typhimurium TA100. In absence of VDC the enzymatic activity declined gradually to nil after 14 h of incubation at 37 degrees C. The presence of EDTA greatly enhanced the microsome-mediated mutagenicity of VDC and led to prolonged enzymatic viability, but only when liver fractions from phenobarbitone (PB) pretreated mice were used. The efficiency of the plate incorporation assay for the detection of mutagens is discussed in comparison with assays in liquid suspension.
在沙门氏菌/微粒体诱变性试验中，对影响小鼠肝脏微粒体混合功能氧化酶将偏二氯乙烯（1,1 - 二氯乙烯）（VDC）转化为诱变剂的酶活性的因素进行了研究。掺入软琼脂层的微粒体部分在7小时内以恒定速率将VDC转化为诱变剂；用鼠伤寒沙门氏菌TA100检测到这些诱变剂。在没有VDC的情况下，在37℃孵育14小时后，酶活性逐渐下降至零。EDTA的存在极大地增强了微粒体介导的VDC诱变性，并导致酶活性延长，但仅在使用苯巴比妥（PB）预处理小鼠的肝脏部分时才会出现这种情况。与液体悬浮试验相比，讨论了平板掺入试验检测诱变剂的效率。
