Liu Yilin, Wan Lingfeng, Zhang Danhong, Ma Yibo, Hu Danni, Li Hongdong, Yao Chunli
Department of the Dermatology, The Second Hospital of Jilin University, Changchun 130012, PR China.
State Key Lab of Superhard Materials, Jilin University, Changchun 130012, PR China.
Bioelectrochemistry. 2025 Dec;166:109052. doi: 10.1016/j.bioelechem.2025.109052. Epub 2025 Jul 19.
Bleomycin (BLM), an essential antitumour medication in clinical applications, has detrimental effects on human health when it appears in an ecological environment, even at trace concentrations. It is desirable to detect trace amounts of BLM by a fast, simple, and efficient method, however, the traditional technologies (e.g., high-performance liquid chromatography, enzyme-linked immunosorbent assay, etc.) generally require expensive equipment and a complicated process, and/or have the disadvantages of long-time running times and poor repeatability. Electrochemical biosensors have been proposed as a fast, easy, sensitive, and cost-effective method for the detection of BLM, and finding a suitable work electrode plays a crucial role in realizing highly performance detection.
In this work, based on a boron-doped diamond (BDD) electrode, coupling with gold (Au) nanoparticles, we design an electrochemical aptasensor for sensitive and specific detection of BLM, featuring a synergistic assembly of aptamers, Au nanoparticles, and BDD film. The BLM aptasensor presents a wide linear range in 1.0 × 10-1.0 × 10 mol L, and achieves a limit of detection as low as 8.5 × 10 mol L. The examinations of trace BLM serum also present a large region of 1.0 × 10-1.0 × 10 mol L. Besides, the interfering tests exibit that the relative responses (RR, %) are ranged from -4.1 % to 6.4 % in five similar structure interfer, and the daily RRs varies between between -3.3 % and 7.3 % in comparison to the 100 % for the first day, which domenstrating the high stability and repeatability and accuracy of the aptasensor.
The innovative aptasensor consisting of β-mercaptohexanol (MCH), Aptamers, Au-NPs, and BDD (MAA-BDD) for the electrochemical detection of trace BLM is of excellent sensitivity, specificity, rigidity, and repeatability, which is suitable for further practical applications on trace BLM detection in various environments.
博来霉素（BLM）是临床应用中的一种重要抗肿瘤药物，当它出现在生态环境中时，即使是痕量浓度，也会对人类健康产生有害影响。期望通过一种快速、简单且高效的方法检测痕量的BLM，然而，传统技术（如高效液相色谱法、酶联免疫吸附测定法等）通常需要昂贵的设备和复杂的过程，和/或存在运行时间长和重复性差的缺点。电化学生物传感器已被提出作为一种快速、简便、灵敏且经济高效的检测BLM的方法，并且找到合适的工作电极对于实现高性能检测起着至关重要的作用。
在这项工作中，基于硼掺杂金刚石（BDD）电极，结合金（Au）纳米粒子，我们设计了一种用于灵敏且特异性检测BLM的电化学生物传感器，其具有适体、Au纳米粒子和BDD膜的协同组装。该BLM生物传感器在1.0×10⁻¹.⁰×10⁻⁶mol/L范围内呈现宽线性范围，并实现了低至8.5×10⁻⁸mol/L的检测限。痕量BLM血清检测也呈现出1.0×10⁻⁶⁻1.0×10⁻⁹mol/L的大区域。此外，干扰测试表明，在五种结构相似的干扰物中，相对响应（RR，%）范围为-4.1%至6.4%，与第一天的100%相比，每日RR在-3.3%至7.3%之间变化，这证明了该生物传感器具有高稳定性、重复性和准确性。
由β-巯基己醇（MCH）、适体、Au-NPs和BDD（MAA-BDD）组成的用于电化学检测痕量BLM的创新型生物传感器具有出色的灵敏度、特异性、刚性和重复性，适用于在各种环境中对痕量BLM检测的进一步实际应用。
