Tao Xinlei, Hu Ge, Wang Zixu, Chen Yaoxing, Cao Jing
Laboratory of Anatomy of Domestic Animal, State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
College of Animal Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
Poult Sci. 2025 Jun 28;104(10):105499. doi: 10.1016/j.psj.2025.105499.
The maintenance of intestinal homeostasis relies on the continuous self-renewal and differentiation of intestinal stem cells (ISC). This study examines the effects of monochromatic light on the self-renewal and differentiation of ISC, thereby elucidating the mechanisms by which monochromatic light influences the growth and development of broilers. The post-hatching day 0 (P0) Cobb 500 broilers were randomly assigned to white light (WL), red light (RL), green light (GL) and blue light (BL) until P35. The findings indicated that the body weight of broilers in the BL group was significantly higher than that observed in the WL groups, and the BL group exhibited an increase in jejunal villus length, a decrease in crypt depth, and a reduction in the villus to crypt (V/C) ratio. The mRNA levels of Sglt1, Glut1, Slc7a5, Fabp2, sIgA, and Tff3 in the jejunum of the BL group were significantly increased relative to those in the other light groups. Furthermore, there was a notable increase in the number of enterocytes, goblet cells, Paneth cells, and enteroendocrine cells. The mRNA levels of Muc2, Lyz, Alpi and Chga as well as the protein levels of SI, Lyz and Chga were also significantly raised. Additionally, the BL group promoted the expression of PCNA and Lgr5, increased the mRNA levels of Olfm4 and Ccnd1, and elevated the protein levels of Lrp6 and β-catenin, while decreasing the protein level of APC. Meanwhile, BL group resulted in increased mRNA levels of Notch1, Notch2 and Dll4, along with an increase in the protein level of Notch1. Relative to the WL group, the BL group also enhanced the depth of cecal crypt and the number of PCNA immunoreactive cells, as well as increased the mRNA levels of Muc2, Chga, Lgr5, sIgA and Tff3. These results suggest that BL may facilitate the self-renewal and differentiation of ISC and enhance the morphology and function of the intestinal epithelium by activating the Wnt/β-catenin and Notch signaling pathways.
肠道稳态的维持依赖于肠道干细胞（ISC）的持续自我更新和分化。本研究考察单色光对ISC自我更新和分化的影响，从而阐明单色光影响肉鸡生长发育的机制。将出壳第0天（P0）的科宝500肉鸡随机分为白光（WL）、红光（RL）、绿光（GL）和蓝光（BL）组，饲养至P35。结果表明，BL组肉鸡的体重显著高于WL组，且BL组空肠绒毛长度增加、隐窝深度减小、绒毛与隐窝（V/C）比值降低。与其他光照组相比，BL组空肠中Sglt1、Glut1、Slc7a5、Fabp2、sIgA和Tff3的mRNA水平显著升高。此外，肠上皮细胞、杯状细胞、潘氏细胞和肠内分泌细胞的数量显著增加。Muc2、Lyz、Alpi和Chga的mRNA水平以及SI、Lyz和Chga的蛋白水平也显著升高。此外，BL组促进了PCNA和Lgr5的表达，提高了Olfm4和Ccnd1的mRNA水平，升高了Lrp6和β-连环蛋白的蛋白水平，同时降低了APC的蛋白水平。同时，BL组导致Notch1、Notch2和Dll4的mRNA水平升高，以及Notch1的蛋白水平增加。相对于WL组，BL组还增加了盲肠隐窝深度和PCNA免疫反应细胞数量，以及Muc2、Chga、Lgr5、sIgA和Tff3的mRNA水平。这些结果表明，蓝光可能通过激活Wnt/β-连环蛋白和Notch信号通路促进ISC的自我更新和分化，并增强肠道上皮的形态和功能。
