Komatsu Syuuhei, Mizuno Yuya, Kikuchi Akihiko
Department of Materials Science and Technology, Tokyo University of Science 6-3-1 Niijuku, Katsushika, Tokyo 125-8585, Japan.
ACS Appl Bio Mater. 2025 Aug 18;8(8):7261-7269. doi: 10.1021/acsabm.5c00954. Epub 2025 Jul 24.
Recent advances in bone regeneration materials have focused on the development of artificial bone scaffolds incorporating bioactive ions, such as strontium ions, that promote bone formation. Incorporating drug retention and release capabilities into these materials is expected to not only improve bone regeneration efficiency but also provide additional drug-derived benefits. The aim of this study is to synthesize organic-inorganic hybrid capsules with a shell containing strontium salts that can retain and release therapeutic agents. The synthesized temperature-responsive polymer formed coacervate droplets that could encapsulate hydrophobic model drugs at temperatures above the lower critical solution concentration (LCST). After preparing Pickering emulsions by mixing calcium carbonate powder and coacervate droplets in an aqueous solution, the calcium carbonate on the surface was allowed to grow crystals under various solvent conditions to produce (Ca,Sr)CO capsules. The (Ca,Sr)CO capsules released Sr ions from the shell phase and also released the encapsulated hydrophobic drug from the inner coacervate phase. In vitro studies using MC3T3-E1 cells showed that exposure to these capsules increased the expression of osteogenic markers, such as alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN). Combining bone regenrative activity with controlled drug loading and release capabilities, the prepared biomaterials have potential as multifunctional scaffolds for bone regeneration strategies.
骨再生材料的最新进展集中在开发包含生物活性离子（如锶离子）的人工骨支架，这些离子可促进骨形成。将药物保留和释放能力纳入这些材料有望不仅提高骨再生效率，还能提供额外的药物衍生益处。本研究的目的是合成具有含锶盐外壳的有机-无机杂化胶囊，该外壳能够保留和释放治疗剂。合成的温度响应聚合物形成了凝聚层液滴，在高于低临界溶液浓度（LCST）的温度下能够包裹疏水性模型药物。在水溶液中通过将碳酸钙粉末与凝聚层液滴混合制备皮克林乳液后，使表面的碳酸钙在各种溶剂条件下生长晶体，以制备（Ca，Sr）CO胶囊。（Ca，Sr）CO胶囊从壳层释放锶离子，同时也从内部凝聚层释放包裹的疏水性药物。使用MC3T3-E1细胞进行的体外研究表明，接触这些胶囊会增加成骨标志物（如碱性磷酸酶（ALP）、骨桥蛋白（OPN）和骨钙素（OCN））的表达。所制备的生物材料将骨再生活性与可控的药物负载和释放能力相结合，具有作为骨再生策略多功能支架的潜力。
