诃子水提取物中去甲诃子次酸对幽门螺杆菌的体外抗菌活性
In vitro antibacterial activity of neochebulinic acid from aqueous extract of Terminalia chebula Retz against Helicobacter pylori.
作者信息
Ou Ling, Chen Haobo, Hao Yajie, Liu Hengrui, Zhu Zhixiang, Li Qingwei, Feng Zhong, Zhang Guimin, Yao Meicun
机构信息
School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen, 518107, Guangdong, China.
International Pharmaceutical Engineering Lab of Shandong Province, Feixian, 273400, Shandong, China.
出版信息
BMC Complement Med Ther. 2025 Jul 24;25(1):287. doi: 10.1186/s12906-025-04989-6.
BACKGROUND
Neochebulinic acid, a component of , exhibits diverse biological activities. This study aimed to investigate its anti- (HP) activity.
METHODS AND MATERIALS
Through using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy, neochebulinic acid was isolated and identified. Efficacy was assessed via minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays, scanning electron microscopy (SEM), and other assays.
RESULTS
It demonstrated neochebulinic acid’s ability to inhibit HP proliferation, damage bacterial structure, and suppress Cytotoxin-associated gene A (Cag A) protein expression, with high selectivity towards HP. Neochebulinic acid also acts as an anti-adhesive agent, preventing HP adhesion to host cells.
CONCLUSION
These findings suggest its potential as a targeted therapeutic against HP infections, paving the way for novel anti-HP therapies.
GRAPHICAL ABSTRACT
[Image: see text]
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1186/s12906-025-04989-6.
背景
新蛇床子素酸是[某种物质]的一种成分，具有多种生物活性。本研究旨在探究其抗幽门螺杆菌（HP）活性。
方法与材料
通过超高效液相色谱-串联质谱（UPLC-MS/MS）和核磁共振（NMR）光谱法，分离并鉴定了新蛇床子素酸。通过最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测定、扫描电子显微镜（SEM）及其他测定方法评估其疗效。
结果
结果表明新蛇床子素酸具有抑制HP增殖、破坏细菌结构以及抑制细胞毒素相关基因A（Cag A）蛋白表达的能力，对HP具有高度选择性。新蛇床子素酸还可作为抗黏附剂，防止HP黏附于宿主细胞。
结论
这些发现表明其作为针对HP感染的靶向治疗药物具有潜力，为新型抗HP治疗方法铺平了道路。
图形摘要
[图像：见正文]
补充信息
在线版本包含可在10.1186/s12906-025-04989-6获取的补充材料。
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