College of Chemistry and Chemical Engineering, Liaoning Normal Univ., Dalian, P.R. China.
College of Pharmacy, Dalian Medical Univ., Dalian, P.R. China.
J Food Sci. 2019 Jul;84(7):1721-1729. doi: 10.1111/1750-3841.14655. Epub 2019 Jun 17.
The fruit of Terminalia chebula Retz., or Tibet Olive, is widely used as a food supplement in China. It possesses some natural antimicrobial properties; however, its chemical composition and antivirulence effects have not been identified. In this work, 29 compounds were identified from the peel of T. chebula fruit by ultra-high-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry. Both the extract of T. chebula and its phenolic acid, corilagin, showed antivirulent activity against Staphylococcus aureus. Specifically, they inhibited biofilm formation. The half maximal inhibitory concentration was 0.13 and 3.18 µg/mL for the extract and corilagin, respectively, whereas for α-hemolysin secretion, the respective concentrations were 30 and 10 µg/mL. Its mechanism of action may be due to reducing the transcription of genes related to quorum sensing. These genes included staphylococcal accessory regulator A, intercellular adhesion accessory gene regulator A, and RNAIII. These findings provide evidence that this food supplement could be an effective antivirulent with corilagin as its active ingredient. PRACTICAL APPLICATION: Corilagin from the fruit of Terminalia chebula Retz. may be used as an antibacterial for its antivirulent activity against Staphylococcus aureus.
余甘子，又名西藏橄榄，其果实被广泛用作中国的食品补充剂。它具有一些天然的抗菌特性；然而，其化学成分和抗毒力作用尚未确定。在这项工作中，通过超高效液相色谱-串联四极杆飞行时间质谱法从余甘子果皮中鉴定出 29 种化合物。余甘子提取物及其酚酸柯里拉京均对金黄色葡萄球菌表现出抗毒力活性。具体来说，它们抑制生物膜的形成。提取物和柯里拉京的半最大抑制浓度分别为 0.13 和 3.18μg/ml，而对于α-溶血素分泌，相应的浓度分别为 30 和 10μg/ml。其作用机制可能是由于降低与群体感应相关的基因转录。这些基因包括葡萄球菌辅助调节因子 A、细胞间黏附辅助基因调节因子 A 和 RNAIII。这些发现为这种食品补充剂可能是一种有效的抗毒力剂提供了证据，其有效成分是柯里拉京。实际应用：余甘子果实中的柯里拉京可能因其对金黄色葡萄球菌的抗毒力活性而被用作抗菌剂。
