Hegnhøj J, Schaffalitzky de Muckadell O B
Scand J Clin Lab Invest. 1985 Oct;45(6):489-95. doi: 10.3109/00365518509155248.
A sensitive and specific enzyme linked immunoassay for lactoferrin is described. The assay is designed to estimate lactoferrin concentrations in samples of duodenal aspirate, but is suitable for other biological fluids as well. The detection limit was 4.7 pmol/l. Within and between assay reproducibility was 41 and 178 pmol/l (1 SD), respectively, at a concentration of 1974 pmol/l. In the fasting state the concentration of lactoferrin in duodenal aspirates of three normal subjects ranged from 0-390 pmol/l and increased to 2860-14,820 pmol/l after pancreatic stimulation.
本文描述了一种用于乳铁蛋白的灵敏且特异的酶联免疫分析方法。该分析方法旨在估计十二指肠抽吸物样本中的乳铁蛋白浓度，但也适用于其他生物体液。检测限为4.7 pmol/l。在浓度为1974 pmol/l时，分析方法内部和之间的重现性分别为41和178 pmol/l（1个标准差）。在禁食状态下，三名正常受试者十二指肠抽吸物中乳铁蛋白的浓度范围为0 - 390 pmol/l，胰腺刺激后增加至2860 - 14820 pmol/l。
