Chiu Hui, Robie Alice A, Branson Kristin, Vippa Tanvi, Epstein Samantha, Rubin Gerald M, Anderson David J, Schretter Catherine E
Division of Biology and Biological Engineering, Tianqiao and Chrissy Chen Institute for Neuroscience, California Institute of Technology, Pasadena, United States.
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
Elife. 2025 Jul 25;12:RP88598. doi: 10.7554/eLife.88598.
Persistent internal states are important for maintaining survival-promoting behaviors, such as aggression. In female , we have previously shown that individually activating either aIPg or pC1d cell types can induce aggression. Here we investigate further the individual roles of these cholinergic, sexually dimorphic cell types, and the reciprocal connections between them, in generating a persistent aggressive internal state. We find that a brief 30-second optogenetic stimulation of aIPg neurons was sufficient to promote an aggressive internal state lasting at least 10 minutes, whereas similar stimulation of pC1d neurons did not. While we previously showed that stimulation of pC1e alone does not evoke aggression, persistent behavior could be promoted through simultaneous stimulation of pC1d and pC1e, suggesting an unexpected synergy of these cell types in establishing a persistent aggressive state. Neither aIPg nor pC1d show persistent neuronal activity themselves, implying that the persistent internal state is maintained by other mechanisms. Moreover, inactivation of pC1d did not significantly reduce aIPg-evoked persistent aggression, arguing that the aggressive state did not depend on pC1d-aIPg recurrent connectivity. Our results suggest the need for alternative models to explain persistent female aggression.
持久的内部状态对于维持促进生存的行为（如攻击性）很重要。在雌性动物中，我们之前已经表明，单独激活aIPg或pC1d细胞类型都可以诱发攻击性。在这里，我们进一步研究这些胆碱能、具有性别差异的细胞类型的个体作用，以及它们之间的相互连接，在产生持久的攻击性内部状态中的作用。我们发现，对aIPg神经元进行30秒的短暂光遗传学刺激足以促进持续至少10分钟的攻击性内部状态，而对pC1d神经元进行类似刺激则不然。虽然我们之前表明单独刺激pC1e不会引发攻击性，但通过同时刺激pC1d和pC1e可以促进持久行为，这表明这些细胞类型在建立持久攻击性状态方面存在意想不到的协同作用。aIPg和pC1d本身都没有显示出持续的神经元活动，这意味着持久的内部状态是由其他机制维持的。此外，pC1d的失活并没有显著降低aIPg诱发的持久攻击性，这表明攻击性状态并不依赖于pC1d - aIPg的反复连接。我们的结果表明需要替代模型来解释雌性动物持久的攻击性。
