Sun Hebin, Sun Yimeng, Qi Tong, Wang Zhenyu, Zhao Jianlong, Liang Lijuan
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
Biosensors (Basel). 2025 Jul 14;15(7):454. doi: 10.3390/bios15070454.
A novel nanomaterial photoelectrochemical aptamer sensor based on CdS@NiMoS heterojunction nanocomposites was constructed for highly sensitive detection of chloramphenicol (CAP) in antibiotic residues. Through optimization of the material synthesis process, the optimal doping ratio of MoS to Ni (70% MoS and 10% Ni) was identified, which significantly enhanced the photogenerated carrier separation efficiency. In thin-film preparation, comparative analysis of four film-forming methods led to the determination of an optimal process with stability. To achieve highly specific CAP detection, the nanocomposite chip was integrated with nucleic acid aptamer biorecognition elements within a standard three-electrode detection system. Experimental results demonstrated a linear response (R = 0.998) in the 0.1-2 μM concentration range, with a detection limit of 3.69 nM (3σ/S).
构建了一种基于CdS@NiMoS异质结纳米复合材料的新型纳米材料光电化学适体传感器，用于高灵敏度检测抗生素残留中的氯霉素（CAP）。通过优化材料合成工艺，确定了MoS与Ni的最佳掺杂比例（70%MoS和10%Ni），这显著提高了光生载流子的分离效率。在薄膜制备过程中，通过对四种成膜方法的对比分析，确定了一种具有稳定性的最佳工艺。为实现对CAP的高特异性检测，在标准三电极检测系统中，将纳米复合芯片与核酸适体生物识别元件相结合。实验结果表明，在0.1-2μM浓度范围内呈现线性响应（R = 0.998），检测限为3.69 nM（3σ/S）。
