Rafi Shah Ali, Emon Wahiduzzaman, Rafsan Ahmed Afif, Mahmud Russel Reza, Nayan Md Faysal, Eid Mahmoud M A, Rashed Ahmed Nabih Zaki
Department of Electrical & Electronic Engineering, Ahsanullah University of Science and Technology, Dhaka, Bangladesh.
Department of Electrical & Electronic Engineering, Metropolitan University, Sylhet, Bangladesh.
Cell Biochem Biophys. 2025 Mar;83(1):689-715. doi: 10.1007/s12013-024-01503-6. Epub 2024 Sep 11.
This research explores a novel biosensor design that exhibits much higher sensitivity compared to conventional biosensors. The biosensor's uniqueness originated from its innovative structure, which incorporates N-FK51A/Ag/AlON/BlueP materials, as well as its cutting-edge fabrication method. The refractive index component was considered when designing the SPR biosensor, which was developed from the angular analysis of the attenuated total reflection (ATR) approach for cancer detection. For instance, the resonance angle shifts by 15.57 deg when the refractive index changes from 1.360 to 1.401, demonstrating the sensor's responsiveness to variation in the refractive index. The sensitivities for skin (basal), cervical (HeLa), blood (Jurkat), adrenal gland (PC12), and breast (MDA-MB-231 and MCF-7) cancer cells were 197.65, 243.66, 255.36, 302.71, 372.57, and 416.85 deg/RIU, respectively. Also, the detection accuracy (DA), the figure of merit (FoM), and the quality factor were 0.37/deg, 155.94 (deg/RIU), and 26.71 RIU. We also examine the effects of substituting the noble, dielectric, 2D material layer with conventional biosensor materials for six cancers. Each time, the Ag/AION/BlueP layered structure performed best in distinguishing cancer cells from healthy cells. We also study the prism effects. The proposed biosensor, with a RI of 1.29-1.40, has a linear regression coefficient of R of 0.96094.
本研究探索了一种新型生物传感器设计，与传统生物传感器相比，其灵敏度要高得多。该生物传感器的独特之处源于其创新结构，该结构包含N-FK51A/Ag/AlON/BlueP材料，以及其前沿的制造方法。在设计SPR生物传感器时考虑了折射率成分，该生物传感器是从用于癌症检测的衰减全反射（ATR）方法的角度分析发展而来的。例如，当折射率从1.360变为1.401时，共振角偏移15.57度，表明该传感器对折射率变化的响应能力。对皮肤（基底）、宫颈（HeLa）、血液（Jurkat）、肾上腺（PC12）和乳腺（MDA-MB-231和MCF-7）癌细胞的灵敏度分别为197.65、243.66、255.36、302.71、372.57和416.85度/RIU。此外，检测准确度（DA）、品质因数（FoM）和质量因子分别为0.37/度、155.94（度/RIU）和26.71 RIU。我们还研究了用传统生物传感器材料替代六种癌症的贵金属、电介质、二维材料层的效果。每次，Ag/AION/BlueP层状结构在区分癌细胞和健康细胞方面表现最佳。我们还研究了棱镜效应。所提出的生物传感器，折射率为1.29 - 1.40，线性回归系数R为0.96094。
