新月柄杆菌自发衍生突变体的分离
Isolation of spontaneously derived mutants of Caulobacter crescentus.
作者信息
Johnson R C, Ely B
出版信息
Genetics. 1977 May;86(1):25-32. doi: 10.1093/genetics/86.1.25.
Abstract
Caulobacter crescentus has a penicillinase which precludes the use of penicillin for mutant enrichment. However, two other antibiotics, fosfomycin and D-cycloserine, can be enrich for C. crescentus mutants. In enrichment procedures for C. crescentus auxotrophs, spontaneously derived mutants occur at a frequency of 5-10% among the survivors of an enrichment procedure. Consequently, large numbers of mutants are readily obtained without any need for mutagenesis. These mutants are heterogeneous both with regard to the type of mutation and to the nutritional requirement. A similar procedure has been used to isolate temperature-sensitive mutants.
摘要
新月柄杆菌有一种青霉素酶，这使得不能使用青霉素来富集突变体。然而，另外两种抗生素，即磷霉素和D-环丝氨酸，可用于富集新月柄杆菌突变体。在新月柄杆菌营养缺陷型的富集程序中，自发产生的突变体在富集程序幸存者中的出现频率为5%-10%。因此，无需诱变就能很容易地获得大量突变体。这些突变体在突变类型和营养需求方面都是异质的。类似的程序已被用于分离温度敏感型突变体。
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