Lee Hyun-Ji, Lee Tae Geol, Doh Il, Lee Sang-Won
Nanobio Measurement Group, Korea Research, Institute of Standards and Science, Daejeon, Republic of Korea.
Department of Biomedical Physics, University of Science and Technology, Daejeon, Republic of Korea.
Commun Eng. 2025 Jul 26;4(1):134. doi: 10.1038/s44172-025-00475-6.
Retinal imaging is essential for diagnosing and treating retinal diseases. However, the lack of standardized and realistic optical phantoms limits the calibration and validation of imaging systems. Here we developed a realistic retinal phantom based on the concept of a super phantom designed to replicate the morphological and functional characteristics of the human retina. The phantom comprises a 13-layered structure, microfluidic channels to emulate vascular networks in the human retina, and fluorescent microbeads to replicate retinal autofluorescence. We validated the measurement of axial resolution, depth range, and field-of-view of optical coherence tomography (OCT) using the phantom. In addition, we confirmed the utility of the phantom across multiple ophthalmic imaging modalities, including OCT, OCT angiography, fundus autofluorescence, fluorescein angiography, and indocyanine green angiography. Imaging systems will be able to enhance the diagnosis and therapeutic monitoring of retinal diseases by standardizing imaging systems with this phantom and improving both the qualitative analysis and the accuracy of quantitative parameters, such as retinal thickness.
视网膜成像对于视网膜疾病的诊断和治疗至关重要。然而，缺乏标准化且逼真的光学模型限制了成像系统的校准和验证。在此，我们基于超级模型的概念开发了一种逼真的视网膜模型，旨在复制人类视网膜的形态和功能特征。该模型包括一个13层结构、用于模拟人类视网膜血管网络的微流体通道以及用于复制视网膜自发荧光的荧光微珠。我们使用该模型验证了光学相干断层扫描（OCT）的轴向分辨率、深度范围和视野的测量。此外，我们证实了该模型在多种眼科成像模式中的实用性，包括OCT、OCT血管造影、眼底自发荧光、荧光素血管造影和吲哚菁绿血管造影。通过使用该模型对成像系统进行标准化，并提高定性分析以及诸如视网膜厚度等定量参数的准确性，成像系统将能够加强对视网膜疾病的诊断和治疗监测。
