Baras Bashayer, Almohaimede Amal, Alshibani Yara, Alzahrani Farah, Alageel Raseel, Weir Michael D, Xu Hockin H K
Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 11432, Saudi Arabia.
Department of Restorative Dental Science, Endodontic Division, College of Dentistry, King Saud University, Riyadh 11432, Saudi Arabia.
Bioengineering (Basel). 2025 Jul 16;12(7):768. doi: 10.3390/bioengineering12070768.
Continued efforts have been made to enhance the antibacterial properties of root canal sealers by adding antimicrobial agents to them. This study aims to investigate the antibacterial effect of 0.15% silver nanoparticles (NAg) and 5% dimethylaminohexadecyl methacrylate (DMAHDM) when added to EndoSequence Bioceramic (BC) sealer against () biofilm and their impact on its physical properties (flowability and film thickness). Four root canal sealers were tested for flow and film thickness properties, as well as against antibiofilm of -impregnated dentin discs, as follows: group 1: EndoSequence BC sealer only; group 2: EndoSequence BC sealer + 0.15% NAg; group 3: EndoSequence BC sealer + 5% DMAHDM; and group 4: EndoSequence BC sealer + 0.15% NAg + 5% DMAHDM. The findings show that all groups had flow and film thickness values that were in accordance with the ISO requirements. Combining 0.15% NAg and 5% DMAHDM in EndoSequence significantly reduced colony-forming unit (CFU) counts by approximately 5 logs. The combination of NAg and DMAHDM offers a promising strategy for developing endodontic sealers with improved antimicrobial properties and acceptable physical performance.
人们一直在不断努力，通过向根管封闭剂中添加抗菌剂来增强其抗菌性能。本研究旨在调查当向EndoSequence生物陶瓷（BC）封闭剂中添加0.15%的银纳米颗粒（NAg）和5%的甲基丙烯酸十六烷基二甲胺（DMAHDM）时，其对（）生物膜的抗菌效果及其对物理性能（流动性和膜厚度）的影响。对四种根管封闭剂进行了流动性和膜厚度性能测试，以及对含（）的牙本质盘的抗生物膜测试，如下：第1组：仅EndoSequence BC封闭剂；第2组：EndoSequence BC封闭剂 + 0.15% NAg；第3组：EndoSequence BC封闭剂 + 5% DMAHDM；第4组：EndoSequence BC封闭剂 + 0.15% NAg + 5% DMAHDM。研究结果表明，所有组的流动性和膜厚度值均符合ISO要求。在EndoSequence中同时添加0.15% NAg和5% DMAHDM可使菌落形成单位（CFU）计数显著减少约5个对数级。NAg和DMAHDM的组合为开发具有更好抗菌性能和可接受物理性能的牙髓封闭剂提供了一种有前景的策略。
